Undifferentiated stem cell culture systems
First Claim
1. A method of expanding human pluripotent stem cells (SCs), the method comprisingincubating human pluripotent SCs in suspension within a culture system comprising basic medium, a knockout serum replacement (KOSR) and a TGFβ
- superfamily factor,wherein the human pluripotent SCs do not undergo differentiation during the expansion.
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Abstract
The present application discloses methods expanding SCs in an undifferentiated state, the methods comprising incubating undifferentiated SCs in suspension within a culture system comprising basic medium and knockout serum replacement (KOSR). The methods may also be applicable for selective spontaneous or directed differentiation of SCs into a selected population of somatic cells from a culture system of SCs in suspension, the method further comprising incubating said undifferentiated SCs in culture system that support respectively, spontaneous or directed differentiation of SCs into the selected population of somatic cells. The present application also discloses a culture system for expansion of stem cells (SCs) comprising a suspension of undifferentiated stem cells within basic medium and knockout serum replacement (KOSR). The methods and culture system of the invention may be used for large scale production of differentiated cells.
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Citations
20 Claims
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1. A method of expanding human pluripotent stem cells (SCs), the method comprising
incubating human pluripotent SCs in suspension within a culture system comprising basic medium, a knockout serum replacement (KOSR) and a TGFβ - superfamily factor,
wherein the human pluripotent SCs do not undergo differentiation during the expansion. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 17, 18, 19)
- superfamily factor,
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9. A culture system comprising a suspension of human pluripotent stem cells (SCs) in a culture medium comprising a basic medium, knockout serum replacement (KOSR), and a TGFβ
- superfamily factor.
- View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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20. A culture system comprising a suspension of human pluripotent stem cells (SCs) within in a culture medium comprising a basic medium, knockout serum replacement (KOSR), a TGFβ
- superfamily member, and one or more element selected from the group consisting of a member of the FGF family, an extracellular matrix (ECM) component, an antibacterial agent, a non-essential amino acid, a neurotrophin, nicotinamide (NA), a bone morphogenic protein (BMP) antagonist and a serum free medium supplement.
Specification