Methods of use of solid support material for binding biomolecules
First Claim
Patent Images
1. A solid support material having a surface, comprising:
- biomolecule-binding groups bonded to the surface through nonreversible covalent bonds;
an aldehyde-containing biomolecule covalently bonded to one or more biomolecule-binding groups through an —
Ar—
N═
C(H)— and
/or —
Ar—
NHN═
C(H)—
linkage, wherein Ar is an aryl group; and
shielding groups covalently bonded to the surface.
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Abstract
Functionalized solid support material with biomolecule-binding groups and uses thereof, wherein the biomolecule-binding groups include a plurality of aromatic groups, an amine group which bonds to a biomolecule through an aldehyde group, a hydrazine group which bonds to a biomolecule through an aldehyde group, or an alpha,beta-ethylenically or acetylenically unsaturated group with an electron withdrawing group.
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Citations
15 Claims
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1. A solid support material having a surface, comprising:
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biomolecule-binding groups bonded to the surface through nonreversible covalent bonds; an aldehyde-containing biomolecule covalently bonded to one or more biomolecule-binding groups through an —
Ar—
N═
C(H)— and
/or —
Ar—
NHN═
C(H)—
linkage, wherein Ar is an aryl group; andshielding groups covalently bonded to the surface. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A method of preparing a solid support material having a biomolecule attached thereto, the method comprising:
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providing a solid support material comprising a surface; providing a shielding compound comprising a shielding group and a surface-bonding group; providing a biomolecule-binding compound comprising an alpha, beta-ethylenically or acetylenically unsaturated group, an electron withdrawing group, and a surface-bonding group; covalently bonding the shielding group and biomolecule-binding group to the surface of the solid support material through the surface-bonding groups; and contacting the solid support material with an amino-functional biomolecule under conditions effective to covalently bond the biomolecule to one or more biomolecule-binding groups through a carbon-nitrogen bond between the amino group of the biomolecule and the beta position of the alpha,beta-ethylenically unsaturated group. - View Dependent Claims (9, 11, 12, 13, 14, 15)
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10. A method of preparing a solid support material having a biomolecule attached thereto, the method comprising:
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providing nanoparticles, each comprising a surface; providing a shielding compound comprising an organocarboxylate-containing and/or organosulfonate-containing group and a surface-bonding group; providing a biomolecule-binding compound comprising an acrylate group and a surface-bonding group; covalently bonding the organocarboxylate and/or organosulfonate group and acrylate group to the surface of the nanoparticles through the surface-bonding groups; and contacting the nanoparticles with an amino-functional biomolecule under conditions effective to covalently bond the biomolecule to one or more acrylate groups through a carbon-nitrogen bond between the amino group of the biomolecule and the beta position of the acrylate group.
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Specification