Genetic adjuvants for immunotherapy
First Claim
1. A method for modulating an immune response, comprising administering host cells and an antigen to a mammal, wherein the host cells have been obtained from the mammal and have been genetically modified with an expression vector to produce, or increase production of, p35 and p40 subunits of IL-12, and IFN-γ
- , by the host cells, wherein the expression vector comprises a nucleic acid encoding the p35 and p40 subunits of IL-12 and IFN-γ
operably linked to a promoter, wherein the p35 and p40 subunits of IL-12 and IFN-γ
are secreted by the administered host cells in the mammal, and wherein said administering of the host cells and the antigen result in an increase of IFN-γ and
IL-2 production, an increase of IgG2a specific to the antigen, a decrease of IL-4 production, and reduced serum IgE in the mammal.
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Abstract
The present invention pertains to methods and pharmaceutical compositions for modulating an immune response. The method of the present invention involves administration of an effective amount of nucleic acid molecules encoding interleukin-12 (IL-12), interferon-gamma (IFN-γ), or a combination thereof, to a patient in need of such treatment. The pharmaceutical compositions of the invention contain nucleic acid molecules encoding IL-12 and/or IFN-γ and an operably-linked promoter sequence. In another aspect, the present invention concerns expression vectors containing a nucleotide sequence encoding IL-12 and IFN-γ, and an operably-linked promoter sequence. In another aspect, the present invention concerns cells genetically modified with a nucleotide sequence encoding IL-12 and IFN-γ.
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Citations
7 Claims
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1. A method for modulating an immune response, comprising administering host cells and an antigen to a mammal, wherein the host cells have been obtained from the mammal and have been genetically modified with an expression vector to produce, or increase production of, p35 and p40 subunits of IL-12, and IFN-γ
- , by the host cells, wherein the expression vector comprises a nucleic acid encoding the p35 and p40 subunits of IL-12 and IFN-γ
operably linked to a promoter, wherein the p35 and p40 subunits of IL-12 and IFN-γ
are secreted by the administered host cells in the mammal, and wherein said administering of the host cells and the antigen result in an increase of IFN-γ and
IL-2 production, an increase of IgG2a specific to the antigen, a decrease of IL-4 production, and reduced serum IgE in the mammal. - View Dependent Claims (2, 3, 4, 5, 6, 7)
- , by the host cells, wherein the expression vector comprises a nucleic acid encoding the p35 and p40 subunits of IL-12 and IFN-γ
Specification