Isolated adult pluripotent stem cells and methods for isolating and cultivating thereof
First Claim
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1. A composition consisting of isolated pluripotent adult stem cells obtained from an exocrine glandular tissue of a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue, wherein the exocrine glandular tissue originates from a mammal, wherein the process for producing the isolated pluripotent adult stem cells comprises:
- removal of exocrine glandular tissue;
the tissue thus removed is divided in such a gentle way that cell structures in resulting tissue fragments are largely preserved;
the divided tissue is cultured in a culture medium in the absence of additional specific growth factors or differentiation factors, whereby most of the differentiated cells become detached from the stem cells, whereupon the stem cells adhere on a bottom of a tissue culture vessel;
the remaining tissue and nonadherent differentiated cells are largely separated by a first change of medium and the remaining nonadherent cells are separated by additional changes of medium at intervals of about 2 to 3 days; and
isolating the pluripotent adult stem cells,further wherein the isolated pluripotent adult stem cells are capable of differentiating into cell types of all three germ layers in a culture medium that does not contain any additional growth factors or differentiation factors after culturing under spatial conditions which ensure three dimensional contact of the cells.
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Abstract
This invention relates to isolated pluripotent adult stem cells which are obtained from exocrine glandular tissue as well as methods of isolating and culturing these pluripotent stem cells.
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Citations
26 Claims
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1. A composition consisting of isolated pluripotent adult stem cells obtained from an exocrine glandular tissue of a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue, wherein the exocrine glandular tissue originates from a mammal, wherein the process for producing the isolated pluripotent adult stem cells comprises:
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removal of exocrine glandular tissue; the tissue thus removed is divided in such a gentle way that cell structures in resulting tissue fragments are largely preserved; the divided tissue is cultured in a culture medium in the absence of additional specific growth factors or differentiation factors, whereby most of the differentiated cells become detached from the stem cells, whereupon the stem cells adhere on a bottom of a tissue culture vessel; the remaining tissue and nonadherent differentiated cells are largely separated by a first change of medium and the remaining nonadherent cells are separated by additional changes of medium at intervals of about 2 to 3 days; and isolating the pluripotent adult stem cells, further wherein the isolated pluripotent adult stem cells are capable of differentiating into cell types of all three germ layers in a culture medium that does not contain any additional growth factors or differentiation factors after culturing under spatial conditions which ensure three dimensional contact of the cells. - View Dependent Claims (2, 3, 4, 5, 13, 14, 15)
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- 6. A primary stem cell culture obtained from exocrine glandular tissue from a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue of a mammal, wherein living cells from the exocrine glandular tissue present in the culture consist essentially of undifferentiated pluripotent adult stem cells.
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16. A primary stem cell culture obtained from exocrine glandular tissue from a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue of a mammal, wherein living cells from the exocrine glandular tissue present in the culture consist essentially of undifferentiated adult stem cells which are capable of differentiating into cells of all three germ cell layers, wherein the process for producing the isolated pluripotent adult stem cells comprises:
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removal of exocrine glandular tissue; the tissue thus removed is divided in such a gentle way that cell structures in resulting tissue fragments are largely preserved; the divided tissue is cultured in a culture medium in the absence of additional specific growth factors or differentiation factors, whereby most of the differentiated cells become detached from the stem cells, whereupon the stem cells adhere on a bottom of a tissue culture vessel; the remaining tissue and nonadherent differentiated cells are largely separated by a first change of medium and the remaining nonadherent cells are separated by additional changes of medium at intervals of about 2 to 3 days; and isolating the pluripotent adult stem cells, further wherein the isolated pluripotent adult stem cells are capable of differentiating into cell types of all three germ layers in a culture medium that does not contain any additional growth factors or differentiation factors after culturing under spatial conditions which ensure three dimensional contact of the cells. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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23. A composition consisting of isolated pluripotent adult stem cells obtained from an exocrine glandular tissue of a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue, wherein the exocrine glandular tissue originates from a mammal, wherein the process for producing the isolated pluripotent adult stem cells comprises:
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removal of exocrine glandular tissue; the tissue thus removed is divided in such a gentle way that cell structures in resulting tissue fragments are largely preserved; the divided tissue is cultured in an incubation medium in the absence of specific growth factors or differentiation factors, whereby most of the differentiated cells become detached from the stem cells, whereupon the stem cells adhere on a bottom of a tissue culture vessel; the remaining tissue and nonadherent differentiated cells are largely separated by a first change of medium and the remaining nonadherent cells are separated by additional changes of medium at intervals of about 2 to 3 days; and isolating the pluripotent adult stem cells, further wherein the isolated pluripotent adult stem cells are capable of differentiating into cell types of all three germ layers in a culture medium that does not contain any additional growth factors or differentiation factors after culturing under spatial conditions which ensure three dimensional contact of the cells.
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24. A primary stem cell culture obtained from exocrine glandular tissue from a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue of a mammal, wherein living cells from the exocrine glandular tissue present in the culture consist essentially of undifferentiated adult stem cells which are capable of differentiating into cells of all three germ cell layers, wherein the process for producing the isolated pluripotent adult stem cells comprises:
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removal of exocrine glandular tissue; the tissue thus removed is divided in such a gentle way that cell structures in resulting tissue fragments are largely preserved; the divided tissue is cultured in an incubation medium in the absence of specific growth factors or differentiation factors, whereby most of the differentiated cells become detached from the stem cells, whereupon the stem cells adhere on a bottom of a tissue culture vessel; the remaining tissue and nonadherent differentiated cells are largely separated by a first change of medium and the remaining nonadherent cells are separated by additional changes of medium at intervals of about 2 to 3 days; and isolating the pluripotent adult stem cells, further wherein the isolated pluripotent adult stem cells are capable of differentiating into cell types of all three germ layers in a culture medium that does not contain any additional growth factors or differentiation factors after culturing under spatial conditions which ensure three dimensional contact of the cells.
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25. A composition consisting of isolated pluripotent adult stem cells obtained from an exocrine glandular tissue of a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue, wherein the exocrine glandular tissue originates from a mammal, wherein the process for producing the isolated pluripotent adult stem cells comprises:
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removal of exocrine glandular tissue; the tissue thus removed is divided in such a gentle way that cell structures in resulting tissue fragments are largely preserved; the divided tissue is cultured in an incubation medium plus serum in the absence of additional specific growth factors or differentiation factors, whereby most of the differentiated cells become detached from the stem cells, whereupon the stem cells adhere on a bottom of a tissue culture vessel; the remaining tissue and nonadherent differentiated cells are largely separated by a first change of medium and the remaining nonadherent cells are separated by additional changes of medium at intervals of about 2 to 3 days; and isolating the pluripotent adult stem cells, further wherein the isolated pluripotent adult stem cells are capable of differentiating into cell types of all three germ layers in a culture medium that does not contain any additional growth factors or differentiation factors after culturing under spatial conditions which ensure three dimensional contact of the cells.
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26. A primary stem cell culture obtained from exocrine glandular tissue from a salivary gland, a lacrimal gland, a sudoriferous gland, a sebaceous gland and/or gastrointestinal tissue of a mammal, wherein living cells from the exocrine glandular tissue present in the culture consist essentially of undifferentiated adult stem cells which are capable of differentiating into cells of all three germ cell layers, wherein the process for producing the isolated pluripotent adult stem cells comprises:
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removal of exocrine glandular tissue; the tissue thus removed is divided in such a gentle way that cell structures in resulting tissue fragments are largely preserved; the divided tissue is cultured in an incubation medium plus serum in the absence of additional specific growth factors or differentiation factors, whereby most of the differentiated cells become detached from the stem cells, whereupon the stem cells adhere on a bottom of a tissue culture vessel; the remaining tissue and nonadherent differentiated cells are largely separated by a first change of medium and the remaining nonadherent cells are separated by additional changes of medium at intervals of about 2 to 3 days; and isolating the pluripotent adult stem cells, further wherein the isolated pluripotent adult stem cells are capable of differentiating into cell types of all three germ layers in a culture medium that does not contain any additional growth factors or differentiation factors after culturing under spatial conditions which ensure three dimensional contact of the cells.
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Specification