Sensor calibration
First Claim
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1. A method of calibrating a reversible-binding sensor for detecting an analyte, which method comprises:
- varying the temperature of a first calibration solution from a first temperature (T1) to a second temperature (T2) while the first calibration solution is in contact with a sensing region of the sensor, wherein the first temperature (T1) is different than the second temperature (T2);
detecting a sensor output for each of a plurality of temperatures between the first temperature (T1) and the second temperature (T2) during the varying of the temperature;
determining the sensor output for the first calibration solution as a function of temperature;
varying the temperature of a second calibration solution from a third temperature (T3) to a fourth temperature (T4) while the second calibration solution is in contact with the sensing region, the second calibration solution having a concentration of analyte which is different from that of the first calibration solution, wherein the third temperature (T3) is different than the fourth temperature (T4);
detecting a sensor output for each of a plurality of temperatures between the first temperature (T3) and the second temperature (T4) during the varying of the temperature;
determining the sensor output for the second calibration solution as a function of temperature; and
using the determined sensor output for the first calibration solution and the second calibration solution, each as a function of temperature, to calibrate the sensor.
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Abstract
A method of calibrating a reversible-binding sensor for detecting an analyte includes:
- (i) varying the temperature of a first calibration solution from a first temperature (T1) to a second temperature (T2) while the first calibration solution is in contact with a sensing region of the sensor;
- (ii) determining the sensor output for the first calibration solution as a function of temperature;
- (iii) varying the temperature of a second calibration solution from a third temperature (T3) to a fourth temperature (T4) while the second calibration solution is in contact with the sensing region, the second calibration solution having a concentration of analyte which is different from that of the first calibration solution;
- (iv) determining the sensor output for the second calibration solution as a function of temperature; and
- (v) using the determined sensor output from steps (ii) and (iv) to calibrate the sensor.
36 Citations
22 Claims
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1. A method of calibrating a reversible-binding sensor for detecting an analyte, which method comprises:
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varying the temperature of a first calibration solution from a first temperature (T1) to a second temperature (T2) while the first calibration solution is in contact with a sensing region of the sensor, wherein the first temperature (T1) is different than the second temperature (T2); detecting a sensor output for each of a plurality of temperatures between the first temperature (T1) and the second temperature (T2) during the varying of the temperature; determining the sensor output for the first calibration solution as a function of temperature; varying the temperature of a second calibration solution from a third temperature (T3) to a fourth temperature (T4) while the second calibration solution is in contact with the sensing region, the second calibration solution having a concentration of analyte which is different from that of the first calibration solution, wherein the third temperature (T3) is different than the fourth temperature (T4); detecting a sensor output for each of a plurality of temperatures between the first temperature (T3) and the second temperature (T4) during the varying of the temperature; determining the sensor output for the second calibration solution as a function of temperature; and using the determined sensor output for the first calibration solution and the second calibration solution, each as a function of temperature, to calibrate the sensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17)
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16. A method of calibrating a reversible-binding sensor for detecting an analyte, which method comprises:
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(i) varying the temperature of a first calibration solution from a first temperature (T1) to a second temperature (T2) while the first calibration solution is in contact with a sensing region of the sensor; (ii) determining the sensor output for the first calibration solution as a function of temperature; (iii) varying the temperature of a second calibration solution from a third temperature (T3) to a fourth temperature (T4) while the second calibration solution is in contact with the sensing region, the second calibration solution having a concentration of analyte which is different from that of the first calibration solution; (iv) determining the sensor output for the second calibration solution as a function of temperature; and (v) using the determined sensor output from steps (ii) and (iv) to calibrate the sensor, wherein the analyte reversibly binds to the receptor at an analyte;
receptor ratio of 1;
1, and step (ii) comprises;a. fitting two or more temperatures in a temperature range from T1 to T2 to the following equation;
I=I1+s1(T−
Tc)wherein I is the sensor output, T is the temperature, Tc is the nominal calibration temperature, I1 is the sensor output for the first calibration solution at Tc and s1 is a constant; and b. determining s1; step (iv) comprises a. fitting two or more temperatures in a temperature range from T3 to T4 to the following equation;
I=I2+s2(T−
Tc)wherein I, T, and Tc, arc as defined above, I2 is the sensor output for the second calibration solution at Tc, and s2 is a constant; and b. determining s2; step (v) comprises a. determining the values of temperature coefficients α and
β
using the following equations;
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18. A calibration system comprising one or more control modules, wherein the one or more control modules are configured to contact the sensing region of a reversible binding sensor with consecutive calibration solutions;
- monitor or adjust the temperature of the calibration solutions;
detect a sensor output for each of a plurality of different temperatures during the varying of the temperature for each calibration solution; and
determine sensor output of said sensor as a function of temperature for each of said calibration solutions. - View Dependent Claims (19, 20, 21, 22)
- monitor or adjust the temperature of the calibration solutions;
Specification