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Uniform fragmentation of DNA using binding proteins

  • US 8,609,341 B2
  • Filed: 05/16/2012
  • Issued: 12/17/2013
  • Est. Priority Date: 01/25/2008
  • Status: Active Grant
First Claim
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1. A method for sequencing a plurality of polynucleotides comprising:

  • (a) binding a plurality of protection molecules to a plurality of polynucleotides;

    (b) cleaving polynucleotides in the plurality of polynucleotides that are bound to the protection molecules, thereby generating a plurality of polynucleotide fragments of substantially uniform size;

    (c) amplifying polynucleotide fragments of the plurality of polynucleotide fragments on a solid support, thereby producing an array of locations, each location comprising multiple copies of a single polynucleotide fragment of the plurality of polynucleotide fragments; and

    (d) performing paired-end sequencing on the copies of the polynucleotide fragments at a plurality of the locations of the array, wherein the paired end sequencing comprises sequencing a first strand of each of the copies, converting the first strand into a double-stranded template and sequencing a second strand of the double-stranded template.

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