Mapc generation of lung tissue

• US 8,609,412 B2
• Filed: 04/21/2005
• Issued: 12/17/2013
• Est. Priority Date: 08/05/1999
• Status: Active Grant

• Current Assignee: Regents of The University of Minnesota (University of Minnesota)
• Original Assignee: Regents of The University of Minnesota (University of Minnesota)
• Inventors: Panoskaltsis-Mortari, Angela, Blazar, Bruce
• Primary Examiner(s): Lankford, Blaine
• Application Number:
  US11/587,511
• Publication Number:
  US 20080274088A1
• Time in Patent Office: 3,162 Days
• Field of Search: None
• US Class Current: 435/377
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2039/5156 expressing foreign proteins
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/42 Respiratory system, e.g. lungs, bronchi or lung cells
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/105 Insulin-like growth factors [IGF]
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 2501/235 Leukemia inhibitory factor [LIF]
• CPC Class Codes: C12N 2503/00 Use of cells in diagnostics
• CPC Class Codes: C12N 2506/03 from non-embryonic pluripotent stem cells
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 2533/52 Fibronectin; Laminin
• CPC Class Codes: C12N 5/0607 Non-embryonic pluripotent stem cells, e.g. MASC induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0688 Cells from the lungs or the respiratory tract

Abstract

The present invention relates to methods of improving function in lung tissue by administering a population of multipotent adult progenitor cells (“MAPCs”) or differentiated progeny thereof.

Claims

1. A method to produce alveolar type II epithelial cells (1) in vitro by exposing cells (2) in vitro to factors that result in differentiation of said cells (2) to cells with an alveolar type II epithelial phenotype (1), said cells (2) being isolated expanded human multipotent non-embryonic non-germ cells that can differentiate into at least one cell type of each of the endodermal, ectodermal, and mesodermal embryonic lineages and express telomerase, said cells have undergone at least 10-40 cell doublings in culture, so as to produce cells with an alveolar type II epithelial phenotype (1).

2. The method of claim 1 further comprising isolating the differentiated alveolar type II epithelial cells.

3. The method of claim 1 further comprising growing the alveolar type II epithelial cells in culture medium.

4. The method of any of claims 1-3 further comprising mixing an effective therapeutic amount of the alveolar type II epithelial cells with a pharmaceutically acceptable carrier.