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Read-out method and apparatus

  • US 8,611,619 B2
  • Filed: 08/21/2008
  • Issued: 12/17/2013
  • Est. Priority Date: 08/22/2007
  • Status: Active Grant
First Claim
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1. A method for detecting the presence and/or amount of an analyte in a sample by obtaining a reliable read-out from a composite signal represented by a coloured area on a test member, the colour being obtained as a response to the analyte, the method comprising:

  • providing as said test member a read-out substrate in the form of a bibulous strip having at least one response area and at least one marker on said response area, the marker causing a colour change when the analyte in the sample undergoes a reaction;

    applying a liquid sample, blood, plasma, or any other suitable liquid containing an analyte, on said strip, whereby said sample is caused to migrate by capillary forces along the strip, and undergoes a reaction at predefined locations on said strip;

    illuminating the assay strip;

    recording an image of said strip;

    calculating a colour saturation value S for said image using said at least two wavelength ranges;

    the S-value being calculated as

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