Elongated microparticles having an optically detectable code configured to at least one of reflect or filter light

• US 8,614,852 B2
• Filed: 09/07/2010
• Issued: 12/24/2013
• Est. Priority Date: 08/20/2002
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Kersey, Alan D., Moon, John A., Putnam, Martin A.
• Primary Examiner(s): Lavarias, Arnel C
• Application Number:
  US12/876,927
• Publication Number:
  US 20110003394A1
• Time in Patent Office: 1,204 Days
• Field of Search: None
• US Class Current: 359/569
• CPC Class Codes: G02B 5/1857 using exposure or etching means, e.g. holography, photolithography, exposure to electron or ion beams
• CPC Class Codes: G02B 5/203 having holographic or diffractive elements diffraction gratings per se G02B5/18; holographic elements per se G02B5/32; generating the spectrum using diffraction elements G01J3/18
• CPC Class Codes: G03F 7/001 Phase modulating patterns, e.g. refractive index patterns
• CPC Class Codes: G03H 1/18 Particular processing of hologram record carriers, e.g. for obtaining blazed holograms photographic processing in general G03C, G03D
• CPC Class Codes: G03H 1/182 Post-exposure processing, e.g. latensification
• CPC Class Codes: G03H 2230/10 Microhologram not registered to the substrate
• CPC Class Codes: Y10S 359/90 Methods
• CPC Class Codes: Y10T 428/2982 Particulate matter [e.g., sphere, flake, etc.]
• CPC Class Codes: Y10T 428/2991 Coated
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

An encoded microparticle including an optical substrate including a material that permits light to propagate therethrough. The optical substrate has an elongated body that extends in a direction along a central axis. The optical substrate includes an outer region that extends about the central axis. The encoded microparticle also includes an optically detectable code that is disposed within the optical substrate and extends along the central axis. The outer region surrounds the optically detectable code about the central axis. The optically detectable code is readable when the light propagates through the outer region and is at least one of reflected or filtered by the optically detectable code. Said at least one of reflected or filtered light propagates through the outer region to be detected for reading the optically detectable code.

Claims

1. An encoded microparticle comprising:

an optical substrate comprising a material that permits light to propagate therethrough, the optical substrate having an elongated body that extends in a direction along a central axis between first and second ends of the optical substrate, the optical substrate including an outer region that extends about the central axis, the outer region having a side surface that extends between the first and second ends; and

an optically detectable code disposed within the optical substrate and extending along the central axis, the outer region surrounding the optically detectable code about the central axis, the optically detectable code being readable when the light propagates through the outer region and is at least one of reflected or filtered by the optically detectable code, said at least one of reflected or filtered light propagating through the side surface of the outer region to be detected for reading the optically detectable code.

2. The encoded microparticle in accordance with claim 1, wherein the optical substrate includes etched outer surfaces.

3. The encoded microparticle in accordance with claim 1, wherein the optical substrate is shaped from a photolithographic process.

4. The encoded microparticle in accordance with claim 1, wherein the optical substrate has planar sides that extend parallel to the central axis.

5. The encoded microparticle in accordance with claim 4, wherein the planar sides are etched planar sides.

6. The encoded microparticle in accordance with claim 4, wherein the planar sides are shaped from a photolithographic process.

7. The encoded microparticle in accordance with claim 4, wherein the optical substrate is rectangular along the central axis and has a square cross-section.

8. The encoded microparticle in accordance with claim 4, wherein the planar sides include four planar sides that extend parallel to the central axis.

9. The encoded microparticle in accordance with claim 4, wherein said at least one of reflected or filtered light is permitted to propagate through any one of the planar sides.

10. The encoded microparticle in accordance with claim 1, wherein a length of the optically detectable code is less than a length of the optical substrate.

11. The encoded microparticle in accordance with claim 1, wherein the outer region completely surrounds the optically detectable code in a uniform manner about the central axis.

12. The encoded microparticle in accordance with claim 1, wherein the optical substrate comprises silicon dioxide (SiO₂).

13. The encoded microparticle in accordance with claim 1, wherein the optically detectable code comprises a photosensitive material.

14. The encoded microparticle in accordance with claim 1, wherein the optically detectable code is defined by a grating having a periodic or aperiodic variation in at least one of a refractive index and an optical absorption.

15. The encoded microparticle in accordance with claim 1, wherein the optically detectable code is one of written, impressed, embedded, imprinted, etched, grown, and deposited within the optical substrate.

16. The encoded microparticle in accordance with claim 1, wherein the optically detectable code is defined by a diffraction grating.

17. The encoded microparticle in accordance with claim 1, wherein the optically detectable code is formed from dielectric layers.

18. The encoded microparticle in accordance with claim 1, wherein the optical substrate provides a reaction support to be used during a multiplexed experiment.

19. The encoded microparticle in accordance with claim 18 further comprising nucleic acids attached to the optical substrate.

20. The encoded microparticle in accordance with claim 1, wherein the encoded microparticle comprises a plurality of encoded microparticles in which differently encoded microparticles have optically detectable codes that are unique with respect to each other.

21. The encoded microparticle in accordance with claim 1, wherein the side surface through which said at least one of reflected or filtered light propagates extends substantially parallel to the central axis between the first and second ends.