Methods for preparing a skin graft
First Claim
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1. A method for preparing a skin graft, the method comprising the steps of:
- providing a device comprising a frame, a lid, a bottom plate, a cutter plate, and a top plate, wherein said cutter plate is disposed between said top and bottom plates and wherein said bottom, cutter and top plates are fitted in the frame and wherein each plate comprises a plurality of holes and wherein as assembled the holes in the plates are aligned and the cutter plate is movable within the frame, and wherein the lid is adapted for connecting with a vacuum source;
contacting the device to skin;
applying a vacuum to generate a negative pressure within the device to thereby raise a plurality of blisters through the aligned holes;
actuating the cutter plate to disrupt the alignment of the holes and to cut the raised blisters, thereby generating a plurality of skin grafts; and
applying said grafts to a substrate.
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Abstract
The present invention generally relates to methods for preparing a skin graft. In certain embodiments, methods of the invention involve harvesting a plurality of skin grafts from a subject, applying the grafts to a first substrate, stretching the first substrate, and transferring the grafts from the first substrate to at least a second substrate for application to a patient recipient site.
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Citations
20 Claims
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1. A method for preparing a skin graft, the method comprising the steps of:
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providing a device comprising a frame, a lid, a bottom plate, a cutter plate, and a top plate, wherein said cutter plate is disposed between said top and bottom plates and wherein said bottom, cutter and top plates are fitted in the frame and wherein each plate comprises a plurality of holes and wherein as assembled the holes in the plates are aligned and the cutter plate is movable within the frame, and wherein the lid is adapted for connecting with a vacuum source; contacting the device to skin; applying a vacuum to generate a negative pressure within the device to thereby raise a plurality of blisters through the aligned holes; actuating the cutter plate to disrupt the alignment of the holes and to cut the raised blisters, thereby generating a plurality of skin grafts; and applying said grafts to a substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for preparing a skin graft, the method comprising the steps of:
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providing a device comprising a frame, a lid, a bottom plate, a cutter plate, and a top plate, wherein each plate comprises a plurality of holes and wherein the cutter plate is movable within the frame, and wherein the frame includes a vacuum stop and the lid includes a suction hole barb; contacting the device to skin; applying vacuum pressure to thereby raise a plurality of blisters; actuating the cutter plate to cut the raised blisters, thereby generating a plurality of skin grafts; applying said grafts to a first substrate; and transferring said grafts from said first substrate to at least a second substrate for application to a patient recipient site. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification