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PEG-urate oxidase conjugates and use thereof

  • US 8,618,267 B2
  • Filed: 04/08/2011
  • Issued: 12/31/2013
  • Est. Priority Date: 08/06/1998
  • Status: Expired due to Fees
First Claim
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1. A method for isolating a tetrameric form of urate oxidase (uricase) from a solution of purified uricase comprising tetrameric uricase and uricase aggregates, said method comprising:

  • (a) separating the solution into fractions on at least one separation column at a pH between about 9 and about 10.5, wherein said column is selected from the group consisting of an ion-exchange column and a size-exclusion column; and

    (b) recovering from said column one or more fractions that contain isolated tetrameric uricase, wherein greater than 90% of said isolated uricase in said one or more fractions is in a tetrameric form.

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