Substrates, systems and methods for analyzing materials
First Claim
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1. An analytical device, comprising:
- a substrate comprising a first surface and at least a first optical waveguide disposed upon the first surface, wherein the first optical waveguide is optically coupled to two or more originating waveguides disposed upon the first surface; and
an individual analyte disposed sufficiently proximal to the first surface and external to the waveguide to be illuminated by an evanescent field emanating from the waveguide when light is passed through the waveguide, wherein the individual analyte is immobilized on the first surface such that a signal from the individual analyte is optically resolvable from any other signal from any other analyte immobilized on the first surface and illuminated by the evanescent field.
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Abstract
Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses.
173 Citations
23 Claims
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1. An analytical device, comprising:
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a substrate comprising a first surface and at least a first optical waveguide disposed upon the first surface, wherein the first optical waveguide is optically coupled to two or more originating waveguides disposed upon the first surface; and an individual analyte disposed sufficiently proximal to the first surface and external to the waveguide to be illuminated by an evanescent field emanating from the waveguide when light is passed through the waveguide, wherein the individual analyte is immobilized on the first surface such that a signal from the individual analyte is optically resolvable from any other signal from any other analyte immobilized on the first surface and illuminated by the evanescent field. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. An analytical device, comprising:
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a substrate comprising a first surface and an array of optical waveguide cores proximal to the first surface, wherein the optical waveguide cores are optically coupled to two or more originating waveguides disposed proximal to the first surface; a plurality of optically resolvable molecular complexes, each of which is immobilized sufficiently proximal to the first surface to be illuminated by an evanescent field emanating from at least one of the optical waveguide cores when light is passed through said array; and two or more excitation radiation sources optically coupled to the array to provide the evanescent field by passing excitation light into the array. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification