Chemistrode, a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution

• US 8,622,987 B2
• Filed: 06/04/2009
• Issued: 01/07/2014
• Est. Priority Date: 06/04/2008
• Status: Active Grant

• Current Assignee: University of Chicago
• Original Assignee: University of Chicago
• Inventors: Ismagilov, Rustem F., Chen, Delai, Liu, Weishan, Du, Wenbin
• Primary Examiner(s): Hindenburg, Max
• Assistant Examiner(s): DANEGA, RENEE A
• Application Number:
  US12/737,058
• Publication Number:
  US 20110112503A1
• Time in Patent Office: 1,678 Days
• Field of Search: 604/500, 600/573
• US Class Current: 604/500
• CPC Class Codes: B01D 61/00 Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor separation of gases or vapours by diffusion B01D53/22
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B33Y 80/00 Products made by additive manufacturing
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

A method for sampling and/or introducing a matter to an environment comprises introducing a first array of plugs through a first microchannel of a device into an exchange region of the device in which mass transport between the environment and the plug fluid of at least one plug in the first array of plugs occurs and a second array of plugs is formed. The exchange region is in fluid communication with the first microchannel. The method further comprises directing the second array of plugs into a second microchannel downstream of and in fluid communication with the exchange region.

Claims

1. A method for sampling and/or introducing a matter to an environment, comprising:

introducing a first array of plugs through a first microchannel of a device into an exchange region of the device, the exchange region being exposed to a living environment and in which mass transport of a matter between the living environment and the plug fluid of at least one plug in the first array of plugs occurs such that a second array of plugs is formed;

wherein the exchange region is in fluid communication with the first microchannel; and

directing the second array of plugs into a second microchannel downstream of and in fluid communication with the exchange region.

2. The method of claim 1, further comprising analyzing the contents of at least one plug in the second array of plugs.

3. The method of claim 1, further comprising introducing the matter to at least one plug in the first array of plugs in the first microchannel.

4. A method comprising:

introducing a first array of plugs through a first microchannel of a device into an exchange region of the device, the exchange region being exposed to an environment and in which mass transport of a matter between the environment and the plug fluid of at least one plug in the first array of plugs occurs such that a second array of plugs is formed;

wherein the exchange region is in fluid communication with the first microchannel; and

directing the second array of plugs into a second microchannel downstream of and in fluid communication with the exchange region,

wherein the exchange region comprises a membrane disposed between the first array of plugs and the environment, the membrane allowing selective mass transport of the matter between the environment and the plug fluid of at least one plug in the first array of plugs.

5. The method of claim 4, wherein the environment is the interior of a cell, the exterior of a cell, an intracellular space between cells, a tissue culture, a living organism, a community of organisms, a body of water, a soil sample or a gas.

6. A method for sampling and/or introducing a matter to an environment, comprising:

introducing a first array of plugs through a first microchannel of a device into an exchange region of the device, the exchange region being exposed to an environment and in which mass transport of a matter between the environment and at least one plug in the first array of plugs occurs such that at least one biological cell contained within the environment is transported into the plug fluid of at least one plug from the first array of plugs so that a second array of plugs forms,

wherein the exchange region is in fluid communication with the first microchannel; and

analyzing the contents of at least one plug in the second array of plugs after it passes the exchange region.

7. The method of claim 6, wherein the environment comprises biological material.

8. The method of claim 7, wherein the biological material is part of a living organism.

9. The method of claim 7, wherein the biological material is in the circulatory system or the brain.

10. The method of claim 6, wherein the size of the first microchannel, the size of the plugs, the speed of movement of the array of plugs and the size and/or shape of the exchange region in which mass transport between the environment and at least one plug in the first array of plugs occurs are such that the at least one biological cell contained within the environment is transported into the plug fluid of at least one plug from the first array of plugs when the second array of plugs is formed.

11. The method of claim 6, further comprising:

providing a stimulus to the environment, wherein the stimulus is adjusted as a function of a result of the analysis of the contents of at least one plug in the second array of plugs.

12. The method of claim 7, further comprising:

providing a stimulus to the living organism, wherein the stimulus is adjusted as a function of a result of the analysis of the contents of at least one plug in the second array of plugs.

13. The method of claim 12, wherein the stimulus is at least one of a visual, tactile, auditory, olfactory or gustatory stimulus.

14. The method of claim 6, wherein the at least one plug in the first array of plugs comprises the matter, which is introduced to the environment in the exchange region.

15. The method of claim 14, further comprising:

adjusting at least one of the rate, amount and composition of the introduced matter based on a result of the analysis.

16. The method of claim 14, further comprising:

varying the amount, varying the composition, or varying both the composition and amount of the introduced matter across the array of plugs.

17. A method for sampling an aqueous environment, comprising:

introducing a fluid through a first microchannel of a device, wherein the fluid is immiscible with water, into an exchange region of the device, the exchange region being exposed to an aqueous environment and in which mass transport of a matter between the aqueous environment and the fluid occurs such that an array of plugs is formed in the exchange region upon the mass transport of the matter between the aqueous environment and the fluid, the exchange region in fluid communication with the first microchannel;

directing the array of plugs into a second microchannel downstream of and in fluid communication with the exchange region; and

storing, analyzing or both storing and analyzing the contents of at least one of the plugs.

18. The method of claim 17, wherein the fluid is a fluorinated oil.

19. The method of claim 17, wherein the exchange region is a portion of a curved third microchannel that connects the first and second microchannels.

20. The method of claim 17, wherein the exchange region is along the outside of a bent section of a third microchannel that connects the first and second microchannels.

21. The method of claim 17, further comprising:

introducing an aqueous stream through a third microchannel, in fluid communication with the exchange region, towards the exchange region such that at least a portion of the aqueous stream is incorporated into the array of plugs that are formed in the exchange region.

22. A method for introducing a matter to an environment, comprising:

introducing an array of plugs comprising a carrier fluid and at least one plug, wherein the at least one plug comprises a plug fluid and a matter to be introduced, through a first microchannel of a device into an exchange region of the device, the exchange region being exposed to an environment and in which mass transport of the matter between the environment and the at least one plug in the array of plugs occurs wherein the exchange region is in fluid communication with the first microchannel;

directing the carrier fluid into a second microchannel downstream of and in fluid communication with the exchange region, such that the second microchannel is substantially free of plugs; and

directing substantially all of the plug fluid of the array of plugs into the environment.

23. The method of claim 22, wherein the interior surface of the second microchannel is preferentially wetted by the carrier fluid relative to the plug fluid.