Methods and compositions for feeder-free pluripotent stem cell media containing human serum
First Claim
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1. A method of culturing primate pluripotent stem cells comprising:
- culturing the primate pluripotent stem cells in a tissue culture vessel containing a defined culture medium under conditions essentially free of feeder cells such that said stem cells grow in an undifferentiated state, wherein the medium comprises sufficient human serum to promote attachment of the primate pluripotent stem cells to said tissue culture vessel.
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Abstract
The present invention provides compositions and methods for the culture and maintenance of pluripotent stem cells. More particularly, the present invention provides for compositions and methods for culturing, maintaining, growing and stabilizing primate pluripotent stem cells in a feeder-free defined media further comprising human serum, or a soluble attachment component of the human serum, for promoting cell attachment.
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Citations
20 Claims
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1. A method of culturing primate pluripotent stem cells comprising:
- culturing the primate pluripotent stem cells in a tissue culture vessel containing a defined culture medium under conditions essentially free of feeder cells such that said stem cells grow in an undifferentiated state, wherein the medium comprises sufficient human serum to promote attachment of the primate pluripotent stem cells to said tissue culture vessel.
- View Dependent Claims (2, 3, 4, 5, 6, 19)
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7. A method of culturing primate pluripotent stem cells comprising:
- culturing the primate pluripotent stem cells in a matrix free tissue culture vessel containing a defined culture medium under conditions essentially free of feeder cells such that the stem cells grow in an undifferentiated state, wherein the medium further comprising about 10% human serum, sufficient to promote attachment of the primate pluripotent stem cells to the said tissue culture vessel.
- View Dependent Claims (8, 9, 20)
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10. A method of culturing primate pluripotent stem cells comprising:
- culturing the primate pluripotent stem cells in a matrix free tissue culture vessel containing a defined culture medium under conditions essentially free of feeder cells such that said stem cells grow in an undifferentiated state, and wherein the medium comprises a fraction of human serum comprising a soluble attachment activity to promote attachment of the primate pluripotent stem cell to a tissue culture vessel.
- View Dependent Claims (11, 12, 13, 14)
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15. A method of culturing primate pluripotent stem cells comprising:
- culturing the primate pluripotent stem cells in a matrix free tissue culture vessel containing a defined culture medium under conditions essentially free of feeder cells such that said stem cells grow in an undifferentiated state and wherein the culture medium further comprises about 0.5% to about 10% human serum wherein a fraction of the human serum comprises a soluble attachment activity, the culturing step being conducted for over one month with the pluripotent stem cells proliferating in culture while maintaining the potential of the stem cells to differentiate into derivatives of endoderm, mesoderm, and ectoderm tissues, and while maintaining the karyotype of the stem cells.
- View Dependent Claims (16, 17, 18)
Specification