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Single cell nucleic acid analysis

  • US 8,628,923 B2
  • Filed: 01/13/2010
  • Issued: 01/14/2014
  • Est. Priority Date: 01/13/2009
  • Status: Active Grant
First Claim
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1. A method for analyzing DNA from a single cell, said method comprising:

  • (a) performing whole genome amplification of the genome of a single cell to produce an amplified genome, wherein the method entails performing whole genome amplification for fewer than, or about, 10 polymerase chain reaction (PCR) cycles;

    (b) preamplifying the amplified genome using a plurality of target-specific primer pairs to produce a preamplification reaction mixture comprising a plurality of amplicons specific for one or more target nucleic acids;

    (c) distributing the preamplification reaction mixture into separate chambers of a microfluidic device prior to amplification; and

    (d) amplifying and detecting the plurality of amplicons, wherein different amplicons are amplified and detected in separate chambers.

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