Probe and primer for tubercle bacillus detection, and method of detecting human tubercle bacillus therewith
First Claim
1. A method for selectively detecting Mycobacterium tuberculosis and not detecting other Mycobacteria species, comprising using a primer comprising an oligonucleotide comprising a nucleotide sequence selected from SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4 or SEQ ID NO;
5, or the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis.
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Accused Products
Abstract
Primers and probes are provided for selectively detecting tubercle bacillus, and methods for detecting low copy numbers of human tubercle bacillus (Mycobacterium tuberculosis) using the same are presented. Oligonucleotides comprising a part of or an entire sequence of the nucleic acid sequence described in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:S, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8, or a part of or an entire sequence of the complementary sequence thereof are disclosed, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. Primers and probes containing such oligonucleotides, and methods for detecting Mycobacterium tuberculosis using the primers and the probes are presented.
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Citations
20 Claims
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1. A method for selectively detecting Mycobacterium tuberculosis and not detecting other Mycobacteria species, comprising using a primer comprising an oligonucleotide comprising a nucleotide sequence selected from SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4 or SEQ ID NO;
5, or the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
- 1, SEQ ID NO;
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20. A method for selectively detecting Mycobacterium tuberculosis and not detecting other Mycobacteria species, comprising using a probe comprising an oligonucleotide portion consisting of the nucleotide sequence selected from SEQ ID NO:
- 1, SEQ ID NO;
2, SEQ ID NO;
3, SEQ ID NO;
4, SEQ ID NO;
5, SEQ ID NO;
6, SEQ ID NO;
7, SEQ ID NO;
8, or SEQ ID NO;
11, or the complementary sequence thereof, wherein the oligonucleotide has a property of hybridizing with the nucleic acid sequence of IS6110 gene in Mycobacterium tuberculosis.
- 1, SEQ ID NO;
Specification