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Identification of canine proBNP by immunoassay

  • US 8,628,973 B2
  • Filed: 09/08/2005
  • Issued: 01/14/2014
  • Est. Priority Date: 09/08/2004
  • Status: Active Grant
First Claim
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1. A method of diagnosing cardiac insufficiency in a canine by detecting canine proBNP or a fragment thereof containing an epitope in the amino acid sequence of SEQ ID NO:

  • 2, an epitope in the amino acid sequence of SEQ ID NO;

    3, and an epitope in the amino acid sequence of SEQ ID NO;

    5 in a canine sample, the method comprising;

    contacting a canine blood sample with a first antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;

    2 of canine proBNP, a second antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;

    3 of canine proBNP, and a third antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;

    5 of canine proBNP;

    detecting the canine proBNP or a fragment thereof containing an epitope in the amino acid sequence of SEQ ID NO;

    2, an epitope in the amino acid sequence of SEQ ID NO;

    3, and an epitope in the amino acid sequence of SEQ ID NO;

    5 present in the sample by detecting the binding of the antibodies to the epitopes;

    comparing the amount of the canine proBNP or fragment thereof in the sample with the amount of canine proBNP or fragment thereof in a blood sample from a healthy canine; and

    diagnosing cardiac insufficiency in the canine from which the sample was obtained if the amount of the canine proBNP or fragment thereof in the sample is higher than the amount of canine proBNP or fragment thereof in a sample from the healthy canine.

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