Identification of canine proBNP by immunoassay
First Claim
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1. A method of diagnosing cardiac insufficiency in a canine by detecting canine proBNP or a fragment thereof containing an epitope in the amino acid sequence of SEQ ID NO:
- 2, an epitope in the amino acid sequence of SEQ ID NO;
3, and an epitope in the amino acid sequence of SEQ ID NO;
5 in a canine sample, the method comprising;
contacting a canine blood sample with a first antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;
2 of canine proBNP, a second antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;
3 of canine proBNP, and a third antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;
5 of canine proBNP;
detecting the canine proBNP or a fragment thereof containing an epitope in the amino acid sequence of SEQ ID NO;
2, an epitope in the amino acid sequence of SEQ ID NO;
3, and an epitope in the amino acid sequence of SEQ ID NO;
5 present in the sample by detecting the binding of the antibodies to the epitopes;
comparing the amount of the canine proBNP or fragment thereof in the sample with the amount of canine proBNP or fragment thereof in a blood sample from a healthy canine; and
diagnosing cardiac insufficiency in the canine from which the sample was obtained if the amount of the canine proBNP or fragment thereof in the sample is higher than the amount of canine proBNP or fragment thereof in a sample from the healthy canine.
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Abstract
The invention relates to a method of determining feline or canine proBNP or fragments thereof, comprising the steps of
- providing a feline or canine sample,
- contacting the sample with at least one antibody which, when determining feline proBNP, or fragments thereof, respectively, binds to at least one epitope in the region comprising the amino acids 20 to 42 and/or in the region comprising the amino acids 57 to 80 of feline proBNP and, when determining canine proBNP, or fragments thereof, binds to at least one epitope in the region comprising the amino acids 20 to 86 of canine proBNP, and
- determining the presence and/or concentration of the feline or canine proBNP, or fragments thereof, present in the sample.
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Citations
9 Claims
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1. A method of diagnosing cardiac insufficiency in a canine by detecting canine proBNP or a fragment thereof containing an epitope in the amino acid sequence of SEQ ID NO:
- 2, an epitope in the amino acid sequence of SEQ ID NO;
3, and an epitope in the amino acid sequence of SEQ ID NO;
5 in a canine sample, the method comprising;contacting a canine blood sample with a first antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;
2 of canine proBNP, a second antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;
3 of canine proBNP, and a third antibody that binds to an epitope in the amino acid sequence of SEQ ID NO;
5 of canine proBNP;detecting the canine proBNP or a fragment thereof containing an epitope in the amino acid sequence of SEQ ID NO;
2, an epitope in the amino acid sequence of SEQ ID NO;
3, and an epitope in the amino acid sequence of SEQ ID NO;
5 present in the sample by detecting the binding of the antibodies to the epitopes;comparing the amount of the canine proBNP or fragment thereof in the sample with the amount of canine proBNP or fragment thereof in a blood sample from a healthy canine; and diagnosing cardiac insufficiency in the canine from which the sample was obtained if the amount of the canine proBNP or fragment thereof in the sample is higher than the amount of canine proBNP or fragment thereof in a sample from the healthy canine. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- 2, an epitope in the amino acid sequence of SEQ ID NO;
Specification