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Human embryonic stem cells genetically modified to contain a nucleic acid and cultured with fibroblast growth factor

  • US 8,637,311 B2
  • Filed: 07/09/2008
  • Issued: 01/28/2014
  • Est. Priority Date: 10/23/1998
  • Status: Expired due to Fees
First Claim
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1. A method of genetically modifying human embryonic stem (“

  • hES”

    ) cells, comprising;

    a. culturing hES cells essentially feeder free on an extracellular matrix in a non-conditioned nutrient media comprising fibroblast growth factor (FGF); and

    b. introducing a nucleic acid into the hES cells of a) thereby genetically modifying the hES cells.

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