Methods and apparatus for creating particle derivatives of HDL with reduced lipid content
First Claim
1. A method of modifying a protein distribution in a fluid, wherein the protein distribution has a first state, the first state having alpha high density lipoproteins and pre-beta high density lipoproteins, comprising the steps of:
- exposing the fluid to a lipid removing agent wherein the exposure modifies the protein distribution from the first state into a second state, the second state having an increased concentration of pre-beta high density lipoproteins relative to the first state; and
,removing the lipid removing agent from the fluid,wherein the lipid removing agent comprises a combination of sevoflurane with at least one of n-butanol, hexanol, ethanol, isoflurane, diisopropyl ether or trifluoroethane.
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Abstract
The present invention is directed to systems, apparatus and methods for creating derivatives of at least one form of HDL without substantially affecting LDL. These derivatives of HDL are particles with reduced lipid content, particularly reduced cholesterol content. These particles have the capacity to bind cholesterol and are administered to a patient to enhance cellular cholesterol efflux and reduce cholesterol levels in cells, tissues, organs, and blood vessels. The present method is useful for treating atherogenic vascular disease and may be combined with other therapies such as statins, inhibitors of cholesterol absorption, niacin, anti-inflammatories, exercise and dietary restriction.
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Citations
24 Claims
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1. A method of modifying a protein distribution in a fluid, wherein the protein distribution has a first state, the first state having alpha high density lipoproteins and pre-beta high density lipoproteins, comprising the steps of:
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exposing the fluid to a lipid removing agent wherein the exposure modifies the protein distribution from the first state into a second state, the second state having an increased concentration of pre-beta high density lipoproteins relative to the first state; and
,removing the lipid removing agent from the fluid, wherein the lipid removing agent comprises a combination of sevoflurane with at least one of n-butanol, hexanol, ethanol, isoflurane, diisopropyl ether or trifluoroethane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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14. A method of modifying a protein distribution in a fluid, wherein the protein distribution has a first state, the first state having more alpha high density lipoproteins than pre-beta high density lipoproteins, comprising the steps of:
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exposing the fluid to a lipid removing agent wherein the exposure modifies the protein distribution from the first state into a second state, the second state having more pre-beta high density lipoproteins than alpha high density lipoproteins; and
,removing the lipid removing agent from the fluid, wherein the lipid removing agent comprises a C4 to C8 alcohol, a C4 to C8 ether, a fluoroether, trifluoroethane or a combination thereof. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification