Use of plants with reduced levels of de-esterified homogalacturonan in the cell wall or portions thereof for improving the saccharification of plant biomasses
First Claim
1. A method of producing sugars from plant biomass, the method comprising enzymatically hydrolyzing biomass of transgenic plants having a reduced content of de-esterified homogalacturonan (HGA) in the pectins of said plants cell walls and a reduced resistance to saccharification with respect to control plants, wherein said transgenic plants are obtained by transformation with a gene encoding an inhibitor of pectin methyl esterases, wherein the inhibitor of pectin methyl esterases has the amino acid sequence of SEQ ID NO:
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Abstract
The present invention relates to expression in plants of pectinolytic enzymes and of pectin methylesterase inhibitors for increasing the degradability of plant tissues through enzymatic digestion, thus improving saccharification efficiency.
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Citations
4 Claims
- 1. A method of producing sugars from plant biomass, the method comprising enzymatically hydrolyzing biomass of transgenic plants having a reduced content of de-esterified homogalacturonan (HGA) in the pectins of said plants cell walls and a reduced resistance to saccharification with respect to control plants, wherein said transgenic plants are obtained by transformation with a gene encoding an inhibitor of pectin methyl esterases, wherein the inhibitor of pectin methyl esterases has the amino acid sequence of SEQ ID NO:
Specification