Method and apparatus for measuring oxidation-reduction potential

• US 8,641,888 B2
• Filed: 10/24/2012
• Issued: 02/04/2014
• Est. Priority Date: 02/28/2011
• Status: Active Grant

• Current Assignee: Caerus Biotechnologies
• Original Assignee: Luoxis Diagnostics, Inc. (Aytu Biopharma, Inc.)
• Inventors: Rael, Leonard T., Bar-Or, David, Bar-Or, Raphael
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US13/659,696
• Publication Number:
  US 20130043142A1
• Time in Patent Office: 468 Days
• Field of Search: 204/400, 204416-418, 204/435, 205/775, 205/782, 205/785.5, 205/789, 205/792
• US Class Current: 205/792
• CPC Class Codes: G01N 27/301 Reference electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 27/403 Cells and electrode assemblies
• CPC Class Codes: G01N 27/4168 Oxidation-reduction potential, e.g. for chlorination of water water analysis G01N33/18
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

Methods and systems for measuring the oxidation-reduction potential of a fluid sample are provided. The system includes a test strip with a sample chamber adapted to receive a fluid sample. The sample chamber can be associated with a filter membrane. The test strip also includes a reference cell. The oxidation-reduction potential of a fluid sample placed in the sample chamber can be read by a readout device interconnected to a test lead that is in electrical contact with the sample chamber, and a reference lead that is in electrical contact with the reference cell. Electrical contact between a fluid sample placed in the sample chamber and the reference cell can be established by a bridge. The oxidation-reduction potential may be read as an electrical potential between the test lead and the reference lead of the test strip.

Claims

1. An oxidation-reduction potential test device, comprising:

a substrate;

a sample chamber;

a filter, wherein the filter occupies at least a portion of the sample chamber;

a first test lead supported by the substrate, the first test lead including;

a first area extending into the sample chamber;

a second area extending from the sample chamber;

a second test lead supported by the substrate, the second test lead including;

a first area extending into the sample chamber;

a second area extending from the sample chamber;

a reference cell;

a reference lead, including;

a first area in electrical contact with the reference cell;

a second area extending from the reference cell;

a bridge, wherein the bridge includes at least a portion of the filter, and wherein a fluid sample in the sample chamber is place in electrical contact with the reference cell by the bridge;

an overlay, wherein at least a portion of the first test lead, at least a portion of the second test lead, at least a portion of the reference cell, at least a portion of the reference lead, and at least a portion of the bridge are held between the substrate and the overlay when the overlay is interconnected to the substrate, wherein the overlay includes an aperture, and wherein the aperture corresponds to at least a portion of the sample chamber when the overlay is interconnected to the substrate;

a filter element, wherein the filter element at least one of extends over and is contained within the sample chamber.

2. A method for determining the oxidation-reduction potential of a fluid sample, comprising:

providing a test strip with a sample chamber, a reference cell, a first test lead, a second test lead, and a reference lead;

placing a fluid sample in a sample chamber, wherein the first test lead, the second test lead, and the reference lead are electrically interconnected to one another via the fluid sample in the sample chamber and via a salt bridge between the sample chamber and the reference cell;

reading a voltage from the sample chamber via at least two of the first test lead, the second test lead, and the reference lead, wherein the reference lead is interconnected to the sample chamber by the reference cell and the bridge.

3. The method of claim 2, further comprising:

providing a first filter element extending between the sample chamber and the reference cell, wherein the bridge is formed from wetting of the filter element by a component of the fluid sample.

4. The method of claim 3, further comprising:

providing a second filter element overlaying the sample chamber, wherein placing the fluid sample in the sample chamber includes filtering the fluid using the second filter element.

5. The method of claim 3, wherein the first filter element overlays the sample chamber, and wherein placing the fluid sample in the sample chamber includes filtering the fluid using the first filter element.

6. The method of claim 2, further comprising:

providing a gel volume that is in communication with the sample chamber, wherein a gel within the gel volume electrically interconnects the fluid sample or a component of the fluid sample to the reference cell.

7. The method of claim 2, wherein providing a test strip with a sample chamber, a reference cell, a first test lead, a second test lead, and a reference cell further comprises:

providing a substrate;

providing an overlay;

providing the first test lead, wherein the first test lead has a sample portion, a transfer portion, and a readout portion, and wherein the first test lead is a substantially uniform, electrically conductive material;

providing the second test lead, wherein the second test lead has a sample portion, a transfer portion, and a readout portion, and wherein the second test lead is a substantially uniform, electrically conductive material;

providing a bridging material;

providing the reference cell;

providing the reference lead having a reference cell contact portion electrically interconnected to the reference cell, and a readout portion;

interconnecting the overlay to the substrate, wherein the test lead, the bridging material, and the reference cell are all at least partially held between the overlay and the substrate, wherein one of the substrate and the overlay includes a sample chamber aperture defining a sample chamber, wherein the sample portion of the test lead extends into the sample chamber, and wherein the readout portion of the test lead and a readout portion of the reference lead are accessible to a readout device.

8. The method of claim 2, wherein the fluid sample is whole blood.