Cell culture surface chemistries

• US 8,642,307 B2
• Filed: 05/25/2006
• Issued: 02/04/2014
• Est. Priority Date: 05/25/2006
• Status: Active Grant

• Current Assignee: Nalge NUNC International Corporation (Perspecta, Inc.)
• Original Assignee: Nalge NUNC International Corporation (Perspecta, Inc.)
• Inventors: Cummins, Thomas, Granchelli, Joesph
• Primary Examiner(s): HANLEY, SUSAN MARIE
• Application Number:
  US11/420,267
• Publication Number:
  US 20070275457A1
• Time in Patent Office: 2,812 Days
• Field of Search: 435/174, 435/180, 435/325, 435/289.1
• US Class Current: 435/180
• CPC Class Codes: C12M 23/20 Material Coatings immunocoatings C12M25/00
• CPC Class Codes: C12M 25/06 Plates; Walls; Drawers; Multilayer plates
• CPC Class Codes: C12N 2533/20 Small organic molecules
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A surface supporting growth of cells in culture, the surface prepare to provide hydrophilic moieties exposed thereon for enhancing the attachment of biological materials. The surface contains the hydrophilic moiety as part of an amphipathic molecule also having a hydrophobic region that is anchored or embedded within a polymer substrate, and oriented such that the hydrophilic moieties are exposed on the outer surface of the substrate. In one embodiment the surface is customized to provide qualitative and quantitative moieties to enhance particular cell growth. In one embodiment, the surface is provided on a culture apparatus such as a dish or plate.

Claims

1. An apparatus for the culture of biological materials, the apparatus, comprising:

a nonfibrous substrate for the attachment of biological materials, the substrate comprising a blend of polymer plastic resin and amphipathic alkylamine ethoxylate molecules; and

cells adherent to a surface of the substrate;

wherein the amphipathic alkylamine ethoxylate molecules comprise hydrophilic moieties and a hydrophobic region, the hydrophobic region being embedded within the polymer plastic resin of the substrate away from contact with the cells, and the hydrophilic moieties being exposed on the surface of the substrate.

2. The apparatus of claim 1 wherein the apparatus is produced by coating the polymer plastic resin with the amphipathic alkylamine ethoxylate molecules while the resin is in pellet form to make the blend prior to melting the resin for the purpose of molding the apparatus.

3. The apparatus of claim 1 wherein the apparatus is produced by compounding the polymer plastic resin with the amphipathic alkylamine ethoxylate molecules to form the blend prior to melting the resin for the purpose of molding the apparatus.

4. The apparatus of claim 1 wherein the plastic polymer of the substrate is chosen from polystyrene, polypropylene, glycol-modified polyethylene terephthalate (PETG), polyethylene terephthalate (PET), polycarbonate, or mixtures thereof.

5. The apparatus of claim 1 wherein the amphipathic molecules have been blended with the polymer plastic resin at a proportion of about 1% w/w.

6. The apparatus of claim 1 wherein he amphipathic molecules have been blended with the polymer plastic resin at a proportion of about 0.1% w/w.

7. The apparatus of claim 1 wherein the hydrophobic region is a chain of carbon linked atoms having a length between 10 to 30 carbon atoms.

8. The apparatus of claim 1 wherein the amphipathic molecules do not include fluorocompounds.

9. An apparatus for the culture of biological materials, the apparatus, comprising:

a nonfibrous substrate for the attachment of biological materials, the substrate comprising a blend of polymer plastic resin and amphipathic alkylamine ethoxylate molecules, wherein the amphipathic alkylamine ethoxylate molecules comprise hydrophilic moieties and a hydrophobic region, the hydrophobic region being embedded within the polymer plastic resin of the substrate, and the hydrophilic moieties being exposed on the surface of the substrate, and the surface with exposed hydrophilic moieties having been treated with corona discharge or plasma discharge, and

cells adherent to the surface of the substrate.

10. An apparatus for the culture of biological materials, the apparatus, comprising:

a nonfibrous substrate for the attachment of biological materials, the substrate comprising a blend of polymer plastic resin and amphipathic alkylamine ethoxylate molecules, wherein the amphipathic alkylamine ethoxylate molecules comprise hydrophilic moieties and a hydrophobic region, the hydrophobic region being embedded within the polymer plastic resin of the substrate, and the hydrophilic moieties being exposed on the surface of the substrate, and the surface with exposed hydrophilic moieties having been coated with poly-D-lysine or collagen, and

cells adherent to the surface of the substrate.