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Methods of neural conversion of human embryonic stem cells

  • US 8,642,334 B2
  • Filed: 02/17/2010
  • Issued: 02/04/2014
  • Est. Priority Date: 02/17/2009
  • Status: Active Grant
First Claim
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1. A method for inducing differentiation in stem cells, comprising,a) providing:

  • i) a cell culture comprising human pluripotent stem cells,ii) a first inhibitor of Small Mothers Against Decapentaplegic (SMAD) protein signaling, wherein said first inhibitor is selected from the group consisting of Noggin, a disulfide-linked homodimer of Noggin, Dorsomorphin, LDN-193189, and mixtures thereof, andiii) a second inhibitor of Small Mothers Against Decapentaplegic (SMAD) protein signaling, wherein said second inhibitor is 4-[4-(1,3-benzodioxol-5-yl)-5-(2-pyridinyl)-1H-imidazol-2-yl] benzamide (SB431542), andb) plating said stem cells in a culture medium,c) contacting said plated pluripotent stem cells within 24-36 hours of said plating with said first inhibitor of Small Mothers Against Decapentaplegic (SMAD) protein signaling and said second inhibitor of Small Mothers Against Decapentaplegic (SMAD) protein signaling, andd) inducing differentiation of said contacted pluripotent stem cells into a population of cultured neuroectodermal precursor cells.

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