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Fabrication of vascularized tissue using microfabricated two-dimensional molds

  • US 8,642,336 B2
  • Filed: 05/24/2010
  • Issued: 02/04/2014
  • Est. Priority Date: 04/30/1999
  • Status: Expired due to Term
First Claim
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1. A tissue lamina comprising:

  • (a) a first mold,(b) a semi-permeable membrane,(c) a second mold, and(d) animal cells attached to the first and second molds and the semi-permeable membrane;

    wherein the first and second molds are fastened together such that the semi-permeable membrane is disposed between the first and second molds,wherein the first and second molds have means defining microchannels positioned towards the semi-permeable membrane,andwherein each of the first and second molds and the semi-permeable membrane are comprised of material that is suitable for attachment and culturing of animal cells.

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