Biologic replacement for fibrin clot

• US 8,642,735 B2
• Filed: 04/18/2012
• Issued: 02/04/2014
• Est. Priority Date: 06/22/1999
• Status: Expired due to Term

• Current Assignee: Children's Medical Center Corporation
• Original Assignee: Children's Medical Center Corporation
• Inventors: Murray, Martha M., Murray, Michael F., Marler, Jennifer
• Primary Examiner(s): KAM, CHIH MIN
• Application Number:
  US13/449,449
• Publication Number:
  US 20120201896A1
• Time in Patent Office: 657 Days
• Field of Search: 530/356, 424/530
• US Class Current: 530/356
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 24/102 Collagen
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/3616 Blood, e.g. platelet-rich plasma
• CPC Class Codes: A61L 27/3645 Connective tissue
• CPC Class Codes: A61L 27/365 Bones
• CPC Class Codes: A61L 27/3654 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/3662 Ligaments, tendons
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3843 Connective tissue
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 41/00 Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C08L 89/06 : derived from leather or skin , e.g. gelatin
• CPC Class Codes: Y10S 623/917 : Collagen

Abstract

The invention provides composition and methods for repairing a ruptured anterior cruciate ligament.

Claims

1. A scaffold for repair of a tissue defect, comprising a three-dimensional matrix, adapted to facilitate cell in-growth, wherein said matrix is shaped for implantation into a patient at the site of the tissue defect, wherein said matrix comprises a natural matrix material wherein the natural matrix material is a collagen glycosaminoglycan copolymer comprised of collagen type I and wherein said scaffold further comprises plasma.

2. The scaffold of claim 1, wherein the matrix is in the shape of a solid cylindrical member, a cylindrical member having hollow cavities, a tube, or a flat sheet rolled into a tube.

3. The scaffold of claim 1, wherein the matrix further comprises one or more of growth factors, antibiotics, glycosaminoglycans, extracellular matrix protein, platelets, cytokines, neutralizing agents, crosslinking agents, stem cells, or cell media supplements.

4. The scaffold of claim 3, wherein the one or more growth factors are selected from the group consisting of epidermal growth factor (EGF);

fibroblast growth factor (FGF);

transforming growth factor (TGF); and

platelet derived growth factor (PDGF).

5. The scaffold of claim 3, wherein the one or more extracellular matrix proteins are selected from the group consisting of elastin, laminin, fibronectin and entectin.

6. The scaffold of claim 1 wherein the scaffold is resistant to degradation by synovial fluid.

7. The scaffold of claim 1, wherein the scaffold is freeze dried.

8. The scaffold of claim 1, further comprising a mechanical device that is a suture or a clip for attachment of the scaffold to the site of the tissue defect to aid in the repair.

9. A method of treating a tissue defect in a subject, the method comprising placing the scaffold of claim 1 at the site of the tissue defect in the subject.

10. A scaffold for repair of a tissue defect, comprising a three-dimensional collagen matrix, wherein said collagen matrix comprises a collagen polymer comprised of type I collagen and wherein said scaffold further comprises plasma.

11. The scaffold of claim 10, further comprising a mechanical device that is a suture or a clip for attachment of the scaffold to the site of the tissue defect to aid in the repair.

12. The scaffold of claim 10, wherein the collagen matrix is in the form of or forming a tube or cylinder.

13. The scaffold of claim 10, wherein the collagen matrix further comprises collagen or collagen glycosaminoglycan copolymer.

14. The scaffold of claim 10, wherein the collagen matrix comprises one or more growth factors selected from the group consisting of epidermal growth factor (EGF);

fibroblast growth factor (FGF);

transforming growth factor (TGF); and

platelet derived growth factor (PDGF).

15. The scaffold of claim 10, wherein the collagen matrix comprises one or more extracellular matrix proteins selected from the group consisting of elastin, laminin, fibronectin and entectin.

16. The scaffold of claim 10, wherein the tissue defect is an intra-articular tissue defect.

17. The scaffold of claim 16, wherein the intra-articular tissue defect is a partial or complete tear of a ligament exposed to synovial fluid.

18. The scaffold of claim 17, wherein the ligament is an anterior cruciate ligament (ACL).

19. The scaffold of claim 16, wherein the intra-articular tissue defect is of meniscus, cartilage, labrum, tendon, muscle, or bone.

20. A method of treating a tissue defect in a subject, the method comprising placing the scaffold of claim 10 at the site of the tissue defect in the subject.