Elite event EE-GM3 and methods and kits for identifying such event in biological samples

• US 8,642,748 B2
• Filed: 01/17/2013
• Issued: 02/04/2014
• Est. Priority Date: 11/23/2009
• Status: Active Grant

• Current Assignee: BASF Agricultural Solutions Seed US LLC (BASF SE), MS Technologies LLC
• Original Assignee: Bayer Cropscience NV (Bayer AG), MS Technologies LLC
• Inventors: Mason, Justin Thomas, Lettow, Leslie James, Eby, Mark Alan, Eby, William H., Welz, Gunter, Verhaeghe, Steven, De Beuckeleer, Marc, Habex, Veerle, Ferullo, Jean-Marc
• Primary Examiner(s): Kruse, David H
• Assistant Examiner(s): KOVALENKO, MYKOLA V
• Application Number:
  US13/744,136
• Publication Number:
  US 20130165642A1
• Time in Patent Office: 383 Days
• Field of Search: 800/300
• US Class Current: 536/23.2
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The invention provides for DNA molecules comprising soybean DNA and foreign DNA comprising herbicide tolerance genes.

Claims

1. A nucleic acid molecule comprising the nucleotide sequence of SEQ ID No:

11 from nucleotide position 1452 to nucleotide position 16638 or the complement thereof, or comprising the nucleotide sequence of SEQ ID No;

11 or the complement thereof.

2. A DNA molecule comprising, in order, the following nucleotide sequences:

a) the nucleotide sequence of SEQ ID No;

2 from nucleotide 1 to 1451;

b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No. 1 from nucleotide 6760 to nucleotide 6958;

c) the nucleotide sequence of SEQ ID No;

1 from nucleotide 6874 to nucleotide 7298;

d) the nucleotide sequence of SEQ ID No;

1 from nucleotide 7 to nucleotide 7291;

e) the nucleotide sequence of SEQ ID No;

1 from nucleotide 12 to nucleotide 7265;

f) the nucleotide sequence of SEQ ID No;

3 from nucleotide 217 to nucleotide 240; and

g) the nucleotide sequence of SEQ ID No;

3 from nucleotide 241 to nucleotide 1408.

3. The nucleic acid molecule of 1, wherein said nucleotide sequence comprises SEQ ID No:

11.

4. A nucleic acid molecule comprising the nucleotide sequence of SEQ ID No:

1 or the complement thereof.

5. A DNA molecule comprising the nucleotide sequence of SEQ ID No:

2 from nucleotide 1441 to nucleotide 1462, and the nucleotide sequence of SEQ ID No;

3 from nucleotide 230 to 251, or the complement thereof.

6. The DNA molecule of claim 5, comprising the nucleotide sequence of SEQ ID No:

2 from nucleotide 1431 to nucleotide 1472, and the nucleotide sequence of SEQ ID No;

3 from nucleotide 220 to 261, or the complement of said sequences.

7. A DNA molecule comprising the nucleotide sequence of SEQ ID No:

2 and the nucleotide sequence of SEQ ID No;

3, or the complement of said sequences.

8. A DNA molecule comprising an artificial genetic locus comprising, in order, the following nucleotide sequences:

a) the nucleotide sequence of SEQ ID No;

2 from nucleotide 1 to 1451;

b) the nucleotide sequence of the complement of the nucleotide sequence of SEQ ID No. 1 from nucleotide 6760 to nucleotide 6958;

c) the nucleotide sequence of SEQ ID No;

1 from nucleotide 6874 to nucleotide 7298;

d) the nucleotide sequence of SEQ ID No;

1 from nucleotide 7 to nucleotide 7291;

e) the nucleotide sequence of SEQ ID No;

1 from nucleotide 12 to nucleotide 7265;

f) the nucleotide sequence of SEQ ID No;

3 from nucleotide 217 to nucleotide 240; and

g) the nucleotide sequence of SEQ ID No;

3 from nucleotide 241 to nucleotide 1408.