Hydrogel implant for sensing metabolites in body tissue

• US 8,647,271 B2
• Filed: 05/23/2008
• Issued: 02/11/2014
• Est. Priority Date: 05/24/2007
• Status: Active Grant

• Current Assignee: EyeSense AG
• Original Assignee: EyeSense AG
• Inventors: Mller, Achim, Herbrechtsmeier, Peter, Knuth, Monika, Nikolaus, Katharina
• Primary Examiner(s): Perkey, W B
• Assistant Examiner(s): Smith, Linda B
• Application Number:
  US12/601,524
• Publication Number:
  US 20100331634A1
• Time in Patent Office: 2,090 Days
• Field of Search: 600/309, 600/318, 600/325
• US Class Current: 600/309
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: G01N 2021/773 Porous polymer jacket; Polymer matrix with indicator
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator

Abstract

An implant (110) for detecting at least one analyte (126) in a body fluid, in particular an eye fluid, is proposed. The implant (110) is designed to be implanted in a tissue layer and/or a chamber of an eye of a patient, the implant (110) having a hydrogel matrix (110) with at least one hydrogel (114). The implant (110) also has sensor particles (116) dispersed in the hydrogel matrix (110), the sensor particles (116) having at least one sensor matrix (120) with a sensor matrix material (122) and at least one sensor material (124).

Claims

1. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising a hydrogel matrix with at least one hydrogel and sensor particles dispersed homogeneously in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, the implant further comprising reference components also dispersed homogeneously in the hydrogel matrix, wherein the reference components are at least substantially analyte-invariant.

2. The implant as claimed in claim 1, the sensor material comprising at least one of the following materials:

concanavalin A;

glucose oxidase;

glucose dehydrogenase;

hexokinase;

glucose/galactose-binding protein;

a protein and/or a fragment functionally equivalent to a protein;

a mutant of hexokinase;

a mutant of glucose/galactose-binding protein; and

a borate ester derivative.

3. The implant as claimed in claim 1, the sensor particles comprising a shell that is at least partially permeable to the analyte.

4. The implant as claimed in claim 1, the sensor material comprising at least one of the following materials:

a molecule and/or a group which, in the presence of an analyte, changes at least one chemical or physical property;

a molecule and/or a group where the analyte, on approaching and/or reacting with the molecule and/or the group, changes at least one chemical or physical property; and

a molecule and/or a group where the analyte undergoes a chemical or physical bonding to the molecule and/or the group, which bonding can be detected by a change in at least one chemical or physical property.

5. The implant as claimed in claim 1, the sensor matrix material. comprising at least one of the following materials;

an alginate;

a polysaccharide;

a polysaccharide derivative;

a synthetic polymer;

a copolymer crosslinked covalently or via a hydrogen bond;

an ionically crosslinked polymer or copolymer;

a polyacrylamide;

a copolymer containing an acrylic acid unit;

an acrylic acid derivative; and

a methacrylic acid derivative.

6. The implant as claimed in claim 1, wherein the reference component comprises at least one luminescent component, wherein the luminescence properties of the luminescent component are at least substantially analyte-invariant.

7. The implant as claimed in claim 1, wherein the implant comprises reference particles implanted in the hydrogel matrix, the reference particles comprising the at least one reference component, the reference component comprising, at least one reference matrix material, and wherein the reference component is bonded physically and/or chemically on the surface and/or the interior of the reference matrix material.

8. A method for detecting at least one analyte in a body fluid, comprising implanting an implant according to claim 1 in a body tissue and detecting the at least one analyte using qualitative or quantitative detection.

9. The implant as claimed in claim 1, wherein the implant is for detecting an eye fluid and the implant is designed to be implanted to a chamber of an eye the patient.

10. The implant as claimed in claim 3, the shell being substantially impermeable to the at least one sensor material.

11. The implant as claimed in claim 3, the shell comprising at least one of the following materials:

a crosslinked protein;

a polysaccharide;

a crosslinked polysaccharide;

a hydrogel with a density exceeding the density of the hydrogel of the hydrogel matrix; and

a layer-by-layer coating.

12. The implant as claimed in claim 4, wherein the at least one chemical or physical property is a fluorescence property.

13. The implant as claimed in claim 7, wherein the physical and/or chemical bond comprises at least one of the following bonds:

a covalent bond;

a complex bond;

an ionic interaction and/or an ionic bond.

14. The implant as claimed in claim 7, wherein the reference component is connected to the reference matrix material at least partially by polymerization.

15. The implant as claimed in claim 7, wherein the reference component is at least substantially immobilized physically in the reference matrix material.

16. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising a hydrogel matrix with at least one hydrogel and sensor particles dispersed in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the at least one sensor material has a higher diffusion coefficient in the sensor matrix material than in the hydrogel matrix.

17. The implant as claimed in claim 16, wherein the sensor matrix material has a lower density than the hydrogel matrix.

18. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising a hydrogel matrix with at least one hydrogel and sensor particles dispersed in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the sensor matrix material comprises a hydrogel with a water content that exceeds the water content of the hydrogel of the hydrogel matrix.

19. The implant as claimed in claim 18, wherein the hydrogel of the sensor matrix material has a water content of at least 70 percent by weight.

20. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising hydrogel matrix with at least one hydrogel and sensor particles dispersed in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the hydrogel matrix and the sensor matrix material comprise a chemically identical hydrogel, wherein the hydrogel of the hydrogel matrix has a higher degree of crosslinking than the hydrogel of the sensor matrix material.

21. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising as hydrogel matrix with at least one hydrogel and sensor particles dispersed homogeneously in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the implant has a shaped hydrogel article, the shaped hydrogel article having a substantially fiat, round configuration, the diameter of the shaped hydrogel article being not greater than 10 mm, and wherein the shaped hydrogel article has a surface-volume ratio of at least 5.

22. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising a hydrogel matrix with at least one hydrogel and sensor particles dispersed homogeneously in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the implant has a shaped hydrogel article, the shaped article having a substantially right-angled edge.

23. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising a hydrogel matrix with at least one hydrogel and sensor particles dispersed homogeneously in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the implant has a shaped hydrogel article, the shaped hydrogel article having a substantially flat, round configuration, the diameter of the shaped hydrogel article being not greater than 10 mm, wherein the shaped article has a curve with a radius of curvature of between 5 mm and 20 mm.

24. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising a hydrogel matrix with at least one hydrogel. and sensor particles dispersed homogeneously in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the implant has a shaped hydrogel article, the shaped hydrogel article having a substantially flat, round configuration, the diameter of the shaped hydrogel article being not greater than 10 mm, wherein the edge area of the shaped article has a thickness of not more than 250 micrometers.

25. An implant for detecting at least one analyte in a body fluid, the implant being designed to be implanted in a body tissue of a patient, the implant comprising a hydrogel matrix with at least one hydrogel and sensor particles dispersed homogeneously in the hydrogel matrix, wherein the sensor particles comprise at least one sensor matrix comprising a sensor matrix material and at least one sensor material, wherein the implant has a shaped hydrogel article, the shaped hydrogel article having a substantially flat, round configuration, the diameter of the shaped hydrogel article being not greater than 10 mm, wherein the shaped article has a rounded edge.