System and method for processing samples

• US 8,647,508 B2
• Filed: 12/01/2009
• Issued: 02/11/2014
• Est. Priority Date: 12/19/2008
• Status: Expired due to Fees

• Current Assignee: 3M Innovative Properties Company (3M Company)
• Original Assignee: 3M Innovative Properties Company (3M Company)
• Inventors: Halverson, Kurt J.
• Primary Examiner(s): Reifsnyder, David A
• Application Number:
  US13/131,658
• Publication Number:
  US 20110236924A1
• Time in Patent Office: 1,533 Days
• Field of Search: 436/178, 436/180, 436/809, 435/287.1, 435/287.3, 435/288.4, 422/502, 422/504, 422/506, 210/637, 210/651, 210/787, 210/808, 210/257.2, 210/321.6
• US Class Current: 210/637
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: G01N 1/4077 by other techniques involving separation of suspended solids
• CPC Class Codes: G01N 2001/4088 filtration
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A system and method for processing samples. The system can include a loading chamber, a detection chamber positioned in fluid communication with the loading chamber, and a fluid path defined at least partially by the loading chamber and the detection chamber. The system can further include a filter positioned such that at least one of its inlet and its outlet is positioned in the fluid path. The method can include positioning a sample in the loading chamber, filtering the sample in the fluid path to form a concentrated sample and a filtrate, removing the filtrate from the fluid path at a location upstream of the detection chamber, moving at least a portion of the concentrated sample in the fluid path to the detection chamber, and analyzing at least a portion of the concentrated sample in the detection chamber for an analyte of interest.

Claims

1. A method for processing samples, the method comprising:

providing a loading chamber;

providing a detection chamber positioned in fluid communication with the loading chamber;

providing a fluid path defined at least partially by the loading chamber and the detection chamber;

positioning a sample in the loading chamber;

filtering the sample with a filter positioned in the loading chamber to form a concentrated sample on the filter and a filtrate, the filter having an inlet and an outlet and positioned such that at least one of the inlet and the outlet is positioned in the fluid path, wherein filtering includes applying a pressure differential across the filter;

removing the filtrate from the fluid path at a location upstream of the detection chamber;

moving at least a portion of the concentrated sample from the filter in the fluid path to the detection chamber by centrifugation; and

analyzing the at least a portion of the concentrated sample in the detection chamber for an analyte of interest.

2. The method of claim 1, wherein the inlet of the filter is positioned in the fluid path and the outlet is positioned such that removing the filtrate from the fluid path includes removing the filtrate via the outlet of the filter and occurs as a result of filtering the sample.

3. The method of claim 1, wherein the inlet and the outlet of the filter are positioned in the fluid path, such that the filtrate is removed from the fluid path at a location that is downstream of the filter.

4. The method of claim 1, wherein the fluid path is a primary fluid path and the filter defines a secondary fluid path, and wherein the secondary fluid path is oriented at an angle with respect to the primary fluid path.

5. The method of claim 1, further comprising eluting the concentrated sample from the filter in the fluid path.

6. The method of claim 5, further comprising agitating the loading chamber to facilitate eluting the concentrated sample from the filter.

7. The method of claim 1, wherein moving at least a portion of the concentrated sample in the fluid path includes eluting at least a portion of the concentrated sample off of the filter.

8. The method of claim 1, wherein providing a detection chamber includes providing a plurality of detection chambers positioned in fluid communication with the loading chamber, and wherein moving at least a portion of the concentrated sample in the fluid path to the detection chamber includes moving at least a portion of the concentrated sample in the fluid path to the plurality of detection chambers.

9. The method of claim 8, wherein the fluid path is further defined by a plurality of channels positioned to fluidly couple the plurality of detection chambers with the loading chamber, and wherein moving at least a portion of the concentrated sample from the filter, in the fluid path to the detection chamber includes moving at least a portion of the concentrated sample along the plurality of channels.

10. The method of claim 8, wherein the fluid path is further defined by a primary channel positioned to provide fluid communication between the loading chamber and the plurality of detection chambers, wherein the fluid path is further defined by a plurality of secondary channels positioned to provide fluid communication between the plurality of detection chambers and the primary channel, and wherein moving at least a portion of the concentrated sample from the filter, in the fluid path to the detection chamber includes moving at least a portion of the concentrated sample along at least a portion of the primary channel and at least one secondary channel to at least one detection chamber.

11. The method of claim 1, further comprising inhibiting the at least a portion of the concentrated sample in the detection chamber from moving out of the detection chamber.

12. The method of claim 11, wherein inhibiting the at least a portion of the concentrated sample in the detection chamber from moving out of the detection chamber includes inhibiting by capillary forces.

13. The method of claim 11, wherein inhibiting the at least a portion of the concentrated sample in the detection chamber from moving out of the detection chamber includes positioning at least one of an obstruction and a seal in the fluid path upstream of the detection chamber.

14. The method of claim 1, further comprising sealing at least a portion of the fluid path after moving at least a portion of the concentrated sample in the fluid path to the detection chamber.

15. A system for processing samples, the system comprising:

a loading chamber adapted to receive a sample;

a detection chamber positioned in fluid communication with the loading chamber;

a fluid path defined at least partially by the loading chamber and the detection chamber;

a filter positioned in the loading chamber and having an inlet and an outlet, the filter positioned such that at least one of the inlet and the outlet is positioned in the fluid path, the filter adapted to filter the sample to form a concentrated sample and a filtrate; and

a filtrate outlet positioned such that the filtrate is removed from the fluid path at a location upstream of the detection chamber.

16. The system of claim 15, wherein the filtrate outlet includes the outlet of the filter.

17. The system of claim 15, wherein the inlet and the outlet of the filter are positioned in the fluid path, and wherein the filtrate outlet is positioned at a location that is downstream of the filter.

18. The system of claim 15, further comprising a channel positioned in fluid communication between the loading chamber and the detection chamber, the channel further defining at least a portion of the fluid path.

19. The system of claim 18, wherein at least a portion of the channel includes a surface modification adapted to facilitate movement of the concentrated sample to the detection chamber.

20. The system of claim 18, wherein the detection chamber is one of a plurality of detection chambers positioned in fluid communication with the loading chamber, and wherein the channel is one of a plurality of channels positioned to fluidly couple the plurality of detection chambers with the loading chamber.

21. The system of claim 18, wherein the detection chamber is one of a plurality of detection chambers positioned in fluid communication with the loading chamber, and wherein the channel is a primary channel, and further comprising a plurality of secondary channels positioned to fluidly couple the plurality of detection chambers with the primary channel.

22. The system of claim 18, wherein the detection chamber is one of a plurality of detection chambers positioned in fluid communication with the loading chamber, and wherein the loading chamber is one of a plurality of loading chambers, and wherein at least some of the plurality of detection chambers are positioned in fluid communication with at least one of the plurality of loading chambers.