System and method for processing samples
First Claim
1. A method for processing samples, the method comprising:
- providing a loading chamber;
providing a detection chamber positioned in fluid communication with the loading chamber;
providing a fluid path defined at least partially by the loading chamber and the detection chamber;
positioning a sample in the loading chamber;
filtering the sample with a filter positioned in the loading chamber to form a concentrated sample on the filter and a filtrate, the filter having an inlet and an outlet and positioned such that at least one of the inlet and the outlet is positioned in the fluid path, wherein filtering includes applying a pressure differential across the filter;
removing the filtrate from the fluid path at a location upstream of the detection chamber;
moving at least a portion of the concentrated sample from the filter in the fluid path to the detection chamber by centrifugation; and
analyzing the at least a portion of the concentrated sample in the detection chamber for an analyte of interest.
1 Assignment
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Accused Products
Abstract
A system and method for processing samples. The system can include a loading chamber, a detection chamber positioned in fluid communication with the loading chamber, and a fluid path defined at least partially by the loading chamber and the detection chamber. The system can further include a filter positioned such that at least one of its inlet and its outlet is positioned in the fluid path. The method can include positioning a sample in the loading chamber, filtering the sample in the fluid path to form a concentrated sample and a filtrate, removing the filtrate from the fluid path at a location upstream of the detection chamber, moving at least a portion of the concentrated sample in the fluid path to the detection chamber, and analyzing at least a portion of the concentrated sample in the detection chamber for an analyte of interest.
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Citations
22 Claims
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1. A method for processing samples, the method comprising:
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providing a loading chamber; providing a detection chamber positioned in fluid communication with the loading chamber; providing a fluid path defined at least partially by the loading chamber and the detection chamber; positioning a sample in the loading chamber; filtering the sample with a filter positioned in the loading chamber to form a concentrated sample on the filter and a filtrate, the filter having an inlet and an outlet and positioned such that at least one of the inlet and the outlet is positioned in the fluid path, wherein filtering includes applying a pressure differential across the filter; removing the filtrate from the fluid path at a location upstream of the detection chamber; moving at least a portion of the concentrated sample from the filter in the fluid path to the detection chamber by centrifugation; and analyzing the at least a portion of the concentrated sample in the detection chamber for an analyte of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A system for processing samples, the system comprising:
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a loading chamber adapted to receive a sample; a detection chamber positioned in fluid communication with the loading chamber; a fluid path defined at least partially by the loading chamber and the detection chamber; a filter positioned in the loading chamber and having an inlet and an outlet, the filter positioned such that at least one of the inlet and the outlet is positioned in the fluid path, the filter adapted to filter the sample to form a concentrated sample and a filtrate; and a filtrate outlet positioned such that the filtrate is removed from the fluid path at a location upstream of the detection chamber. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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Specification