Diagnostic gel composition, method for making a diagnostic gel composition

• US 8,647,742 B2
• Filed: 12/28/2009
• Issued: 02/11/2014
• Est. Priority Date: 12/28/2009
• Status: Active Grant

• Current Assignee: Achira Labs Pvt. Ltd.
• Original Assignee: Achira Labs Pvt. Ltd.
• Inventors: Dendukuri, Dhananjaya, Katiyar, Reeta, Sivakumaran, Lakshmi P.
• Primary Examiner(s): KILIMAN, LESZEK B
• Application Number:
  US13/504,038
• Publication Number:
  US 20120258309A1
• Time in Patent Office: 1,506 Days
• Field of Search: 428402-407, 424/9.6, 424/501, 522/6, 522/153, 252/301.35, 106/316
• US Class Current: 428/402
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 428/2982 Particulate matter [e.g., sphere, flake, etc.]
• CPC Class Codes: Y10T 428/2998 including synthetic resin or polymer

Abstract

The invention relates to a diagnostic gel composition for use as a diagnostic element in diagnostic devices. The diagnostic gel composition is derived from a compound having a formula D-Sp-Po wherein D is a diagnostic group; Sp is a hydrophilic spacer group; and Po is a polymerizable group. The diagnostic gel composition of the invention has dimensions ranging from about 250 nanometers to about 1000 micrometers, and a Young'"'"'s modulus ranging from about 10 kilopascals to about 200 kilopascals. The invention also provides method for making the diagnostic gel composition. The method comprises providing a composition comprising a porogen, an initiator and a compound having a formula D-Sp-Po; polymerizing the composition to form a polymerized composition; and washing the polymerized composition to form the diagnostic gel composition.

Claims

1. A diagnostic gel composition having dimensions ranging from about 250 nanometers to about 1000 micrometers, and a Young'"'"'s modulus ranging from about 10 kilopascals to about 200 kilopascals, wherein the diagnostic gel composition is derived from a compound having a formula

D-Sp-Po;

wherein D is a diagnostic group;

Sp is a hydrophilic spacer group; and

Po is a polymerizable group.

2. The diagnostic gel composition of claim 1, wherein D is an antibody produced by the body against a surface antigen on viruses.

3. The diagnostic gel composition of claim 1, wherein Sp is a poly(ethylene glycol) based group.

4. The diagnostic gel composition of claim 1, wherein Po is a vinyl group.

5. The diagnostic gel composition of claim 1, further comprising pores having pore size ranging from about 5 nanometers to about 1000 nanometers.

6. The diagnostic gel composition of claim 1 Po is a vinyl group and Sp is a poly(ethylene glycol) based group.

8. A diagnostic device comprising the diagnostic element of claim 6.

7. A diagnostic element comprising the diagnostic gel composition of 1.

9. A method of making a diagnostic gel composition, the method comprising:

providing a composition comprising a porogen, an initiator and a compound having a formula;

D-Sp-Po;

polymerizing the composition to form a polymerized composition;

washing the polymerized composition to form the diagnostic gel composition;

wherein D is a diagnostic group;

Sp is a hydrophilic spacer group; and

Po is a polymerizable group, and wherein the diagnostic gel composition has a Young'"'"'s modulus ranging from about 10 kilopascals to about 200 kilopascals.

10. The method of claim 9, wherein the polymerizing is by photopolymerization.

11. The method of claim 10, wherein the polymerizing is effected through a photomask.

12. The method of claim 9, wherein the porogen is sodium bicarbonate.

13. The method of claim 9, wherein the initiator is a photoinitiator.

14. The method of claim 9, wherein D is an HIV-antibody specific to a particular antigen.

15. The method of claim 9, wherein D is an aptamer.

16. The method of claim 9, wherein D is an oligonucleotide.

17. The method of claim 9, wherein Sp is a poly(ethylene glycol) based group.

18. The method of claim 9, wherein Po is a vinyl group.

19. The method of claim 9, further comprising washing off the porogen.