Methods of making small volume in vitro analyte sensors
First Claim
1. A method of manufacturing a plurality of electrochemical sensors, the method comprising the steps of:
- forming a plurality of individual working electrodes on a first electrode region of a first substrate;
forming a plurality of individual counter electrodes on a second electrode region of a second substrate;
positioning a spacer layer comprising a release liner on one of the first and the second electrode regions;
following positioning of the spacer layer, removing the release liner to remove a portion of the spacer layer to define sample chamber regions;
overlaying the first substrate with the second substrate to form a layered structure; and
separating the layered structure into a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, and at least one sample chamber region.
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Accused Products
Abstract
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry; and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
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Citations
28 Claims
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1. A method of manufacturing a plurality of electrochemical sensors, the method comprising the steps of:
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forming a plurality of individual working electrodes on a first electrode region of a first substrate; forming a plurality of individual counter electrodes on a second electrode region of a second substrate; positioning a spacer layer comprising a release liner on one of the first and the second electrode regions; following positioning of the spacer layer, removing the release liner to remove a portion of the spacer layer to define sample chamber regions; overlaying the first substrate with the second substrate to form a layered structure; and separating the layered structure into a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, and at least one sample chamber region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of manufacturing a plurality of electrochemical sensors, the method comprising the steps of:
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forming a plurality of individual working electrodes on a first electrode region of a first substrate; forming a plurality of individual counter electrodes on a second electrode region of a second substrate; positioning a spacer layer comprising a release liner on one of the first electrode region and the second electrode regions; following positioning of the spacer layer, removing the release liner to remove a portion of the spacer layer to define sample chamber regions; overlaying the first substrate with the second substrate to form a layered structure; and cutting the layered structure to provide individual electrochemical sensors, each electrochemical sensor comprising at least one working electrode, at least one counter electrode, and at least one sample chamber region, wherein at least one end of the at least one sample chamber region is defined by a cut. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification