Juvenile cartilage composition

• US 8,652,507 B2
• Filed: 12/22/2010
• Issued: 02/18/2014
• Est. Priority Date: 12/11/2003
• Status: Active Grant

• Current Assignee: Zimmer Incorporated (Zimmer Biomet Holdings, Inc.)
• Original Assignee: Zimmer Incorporated (Zimmer Biomet Holdings, Inc.)
• Inventors: Kizer, Neil, Spiro, Robert, Yao, Jian, Blanchard, Cheryl Renee
• Primary Examiner(s): AZPURU, CARLOS A
• Application Number:
  US12/976,711
• Publication Number:
  US 20120009224A1
• Time in Patent Office: 1,154 Days
• Field of Search: None
• US Class Current: 424/426
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61F 2002/30764 Cartilage harvest sites
• CPC Class Codes: A61F 2002/30766 Scaffolds for cartilage ingrowth and regeneration
• CPC Class Codes: A61F 2310/00293 containing a phosphorus-containing compound, e.g. apatite
• CPC Class Codes: A61F 2310/00365 Proteins; Polypeptides; Degradation products thereof
• CPC Class Codes: A61F 2310/00377 Fibrin
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 27/3612 Cartilage, synovial fluid
• CPC Class Codes: A61L 27/3654 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3817 Cartilage-forming cells, e.g. pre-chondrocytes
• CPC Class Codes: A61L 27/3852 Cartilage, e.g. meniscus
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: C12N 2533/56 Fibrin; Thrombin
• CPC Class Codes: C12N 2533/80 Hyaluronan
• CPC Class Codes: C12N 5/0655 : Chondrocytes; Cartilage

Abstract

The present invention is directed to compositions having at least one neocartilage particle, juvenile cartilage particle or a combination thereof and a matrix, and methods and devices that include the compositions.

Claims

1. A cartilage treatment composition comprising:

cadaveric, allogenic human juvenile cartilage tissue particles comprising viable chondrocytes; and

a matrix, wherein the matrix is defined as a chondro-conductive media;

wherein the chondro-conductive media is a media comprising one or more growth factors.

2. The composition of claim 1, wherein the chondro-conductive media is a media suitable for tissue or cell culture.

3. The composition of claim 2, wherein the chondro-conductive media is at least one of conditioned media, Dulbecco'"'"'s Modified Eagle'"'"'s Medium, Minimal Essential Medium, RPMI medium, media having one or more growth factors, media having ascorbate, media having fibrinogen, or media having thrombin.

4. The composition of claim 3, wherein the chondro-conductive media is at least one of Dulbecco'"'"'s Modified Eagle'"'"'s Medium, Minimal Essential Medium or RPMI medium.

5. The composition of claim 2, wherein the chondro-conductive media is a media having ascorbate.

6. The composition of claim 1, wherein the juvenile cartilage particles are from cadaveric juvenile donors less than fifteen years of age.

7. The composition of claim 6, wherein the juvenile cartilage particles are from cadaveric juvenile donors less than two years of age.

8. The composition of claim 6, wherein the juvenile cartilage particles are from cadaveric juvenile donors from about 20 weeks to about 13 years of age.

9. The composition of any one of claims 1, 2 or 8, wherein the juvenile cartilage particles have a dimension from about one to about three millimeters.

10. The composition of any one of claims 1, 2 or 8, wherein the juvenile cartilage particles range in size from about 1 to about 27 mm³.

11. The composition of any one of claims 1, 2 or 8, wherein the juvenile cartilage particles comprise at least 1×

10⁶ chondrocytes.

12. The composition of any one of claims 1, 2 or 8, comprising at least 60 mg of juvenile cartilage particles.

13. The composition of any one of claims 1, 2 or 8, wherein the cartilage particles comprise articular cartilage.

14. The composition of claim 9, wherein the articular cartilage is harvested from a femoral condyle, a tibial plateau or an interior surface of a patella.

15. The composition of claim 2, wherein the chondro-conductive media is a media having fibrinogen.

16. The composition of claim 2, wherein the chondro-conductive media is a media having thrombin.

17. The composition of claim 2, wherein the chondro-conductive media is a conditioned media.