Recombinant Thermoascus aurantiacus β-glucosidase variants
First Claim
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1. A recombinant variant β
- -glucosidase, comprising a substitution set selected from the group consisting of;
T151S-Y642N-N651K, K101R-T151S-K343R-N356S-S409N-Y642N-N651K, M1T-K55R-K101R-T151S-R331K-Y332C-K343R-N356S-S409N-Y642N, M1T-K101R-T151S-K292E-K343R-S409N-Y642N-P740S, M1T-T151S-K343R-S409N-A479V-Y642N-Y680F, L150V-T151S-K343R-S409N-K457R-Y642N-N651K, and S87N-T151S-F288Y-Y642N-N651K of SEQ ID NO;
4, wherein said recombinant variant β
-glucosidase has greater enzymatic activity than the amino acid sequence of SEQ ID NO;
4.
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Abstract
The present invention provides compositions and methods for the expression of recombinant β-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
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Citations
6 Claims
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1. A recombinant variant β
- -glucosidase, comprising a substitution set selected from the group consisting of;
T151S-Y642N-N651K, K101R-T151S-K343R-N356S-S409N-Y642N-N651K, M1T-K55R-K101R-T151S-R331K-Y332C-K343R-N356S-S409N-Y642N, M1T-K101R-T151S-K292E-K343R-S409N-Y642N-P740S, M1T-T151S-K343R-S409N-A479V-Y642N-Y680F, L150V-T151S-K343R-S409N-K457R-Y642N-N651K, and S87N-T151S-F288Y-Y642N-N651K of SEQ ID NO;
4, wherein said recombinant variant β
-glucosidase has greater enzymatic activity than the amino acid sequence of SEQ ID NO;
4. - View Dependent Claims (2, 3, 4, 5, 6)
- -glucosidase, comprising a substitution set selected from the group consisting of;
Specification