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Recombinant Thermoascus aurantiacus β-glucosidase variants

  • US 8,652,813 B2
  • Filed: 11/19/2010
  • Issued: 02/18/2014
  • Est. Priority Date: 11/25/2009
  • Status: Expired due to Fees
First Claim
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1. A recombinant variant β

  • -glucosidase, comprising a substitution set selected from the group consisting of;

    T151S-Y642N-N651K, K101R-T151S-K343R-N356S-S409N-Y642N-N651K, M1T-K55R-K101R-T151S-R331K-Y332C-K343R-N356S-S409N-Y642N, M1T-K101R-T151S-K292E-K343R-S409N-Y642N-P740S, M1T-T151S-K343R-S409N-A479V-Y642N-Y680F, L150V-T151S-K343R-S409N-K457R-Y642N-N651K, and S87N-T151S-F288Y-Y642N-N651K of SEQ ID NO;

    4, wherein said recombinant variant β

    -glucosidase has greater enzymatic activity than the amino acid sequence of SEQ ID NO;

    4.

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