Compositions, methods and related uses for cleaving modified DNA
First Claim
1. A composition comprising a plurality of similar sized double-stranded fragments, the fragments further characterized by:
- (a) staggered ends; and
(b) a centrally positioned methylated or hydroxymethylated cytosine proximal to a guanine;
wherein the fragments are obtainable by in vitro enzymatic cleavage of a large DNA, the large DNA comprising genomic DNA.
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Accused Products
Abstract
Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N-terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.
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Citations
10 Claims
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1. A composition comprising a plurality of similar sized double-stranded fragments, the fragments further characterized by:
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(a) staggered ends; and (b) a centrally positioned methylated or hydroxymethylated cytosine proximal to a guanine; wherein the fragments are obtainable by in vitro enzymatic cleavage of a large DNA, the large DNA comprising genomic DNA. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification