Biosensor for electrochemical measurement of 1,5-anhydroglucitol, and measuring method and measuring kit using the same
First Claim
1. A biosensor having an electrode system for electrochemically measuring 1,5-anhydroglucitol and a reagent layer formed on the electrode system, wherein the reagent layer comprises:
- (1) a 1,5-anhydroglucitol-measuring enzyme;
(2) a phenothiazine compound;
(3) a stabilizer consisting of one or two or more compounds selected from the group consisting of sodium chloride, sodium citrate, magnesium sulfate, piperazin-1,4-bis(2-ethanesulfonic acid), o-sulfobenzoic acid cyclic, arginine or a salt thereof, glutamic acid or a salt thereof, and lysine or a salt thereof; and
(4) an acidic polymer compound as an optional ingredient.
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Abstract
The biosensor has an electrode system for electrochemically measuring 1,5-anhydroglucitol (1,5-AG) and a reagent layer formed on said electrode system. The reagent layer contains an enzyme for measuring 1,5-anhydroglucitol, phenothiazine compounds, a stabilizer selected from a group of compounds comprising metal salts, organic acids, and amino acids, and an acidic polymer compound as an optional ingredient. The biosensor has excellent storage stability and can electrochemically measure 1,5-anhydroglucitol unaffected by the hematocrit contained in a whole blood sample.
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Citations
9 Claims
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1. A biosensor having an electrode system for electrochemically measuring 1,5-anhydroglucitol and a reagent layer formed on the electrode system, wherein the reagent layer comprises:
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(1) a 1,5-anhydroglucitol-measuring enzyme; (2) a phenothiazine compound; (3) a stabilizer consisting of one or two or more compounds selected from the group consisting of sodium chloride, sodium citrate, magnesium sulfate, piperazin-1,4-bis(2-ethanesulfonic acid), o-sulfobenzoic acid cyclic, arginine or a salt thereof, glutamic acid or a salt thereof, and lysine or a salt thereof; and (4) an acidic polymer compound as an optional ingredient. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification