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Microfluidic particle-analysis systems

  • US 8,658,418 B2
  • Filed: 07/13/2009
  • Issued: 02/25/2014
  • Est. Priority Date: 04/01/2002
  • Status: Expired due to Term
First Claim
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1. A microfluidic device for exposing cells to a plurality of compounds comprising:

  • (a) a cell inlet;

    (b) a plurality of cell chambers in fluidic communication with said cell inlet and at least one of the other cell chambers;

    (c) a plurality of chamber isolation valves, said isolation valves, when actuated, fluidically isolating said chambers from each other;

    (d) a plurality of compound inlets and compound outlets, wherein each chamber is in fluidic communication with a compound inlet and a compound outlet, wherein each chamber includes a channel sized for retaining a single cell within said chamber;

    (e) a shared waste receptacle, said shared waste receptacle being configured to receive waste from said compound outlets;

    (f) a shared reagent reservoir, said shared reagent reservoir being configured to provide reagent to said chambers;

    (g) a plurality of compound outlet valves, said compound outlet valves configured to fluidically isolate said chambers from said shared waste receptacle and said shared reagent reservoir when closed;

    (h) a plurality of analysis sites, wherein each cell chamber has a corresponding analysis site, wherein each chamber is in fluidic communication with a respective analysis site via its channel;

    wherein the plurality of cell chambers includes a first cell chamber and a second cell chamber downstream from the first cell chamber,wherein the first cell chamber is configured to hold a first cell using a retaining force fluidically applied via its channel and thereby allow a second cell following the first cell to be diverted downstream along a shared cell input channel to the second cell chamber.

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