Selective extraction of proteins from freshwater algae
First Claim
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1. A method of selectively removing algal proteins from a wet freshwater algal biomass comprising intact algal cells or wet freshwater algal culture comprising intact algal cells, the method comprising:
- a. harvesting algae from the wet freshwater algal biomass comprising intact algal cells or wet freshwater algal culture comprising intact algal cells;
b. dewatering the harvested algae to remove at least a portion of extracellular water from the harvested algae, to yield a first biomass fraction comprising intact algal cells;
c. extracting said first biomass fraction comprising intact algal cells with a first solvent, yielding a second biomass fraction comprising intact algal cells and a first liquid phase;
d. separating said second biomass fraction comprising intact algal cells from said first liquid phase; and
e. concentrating said first liquid phase to yield a mixture enriched in algal proteins.
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Abstract
Methods for selective extraction and fractionation of algal proteins from an algal biomass or algal culture are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which allow for efficient separation of algal proteins. These proteins can be used as renewable sources of proteins for animal feedstocks and human food. Further, lipids remaining in the algal biomass after extraction of proteins can be used to generate renewable fuels.
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10 Claims
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1. A method of selectively removing algal proteins from a wet freshwater algal biomass comprising intact algal cells or wet freshwater algal culture comprising intact algal cells, the method comprising:
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a. harvesting algae from the wet freshwater algal biomass comprising intact algal cells or wet freshwater algal culture comprising intact algal cells; b. dewatering the harvested algae to remove at least a portion of extracellular water from the harvested algae, to yield a first biomass fraction comprising intact algal cells; c. extracting said first biomass fraction comprising intact algal cells with a first solvent, yielding a second biomass fraction comprising intact algal cells and a first liquid phase; d. separating said second biomass fraction comprising intact algal cells from said first liquid phase; and e. concentrating said first liquid phase to yield a mixture enriched in algal proteins. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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