Device and method for restoration of the condition of blood
First Claim
1. A device for extracorporeal removal of a harmful agent having a binding affinity to heparin from blood or blood components, the device comprising a full length heparin immobilized on a solid substrate by a covalent end point attachment, where said covalent end point attachment is formed by a process comprising the steps of:
- a) providing said solid substrate having primary amino functional groups,b) mixing said solid substrate of a) with said full length heparin and a reducing agent in an aqueous medium,c) allowing said full length heparin to bind covalently to the amino functional groups, wherein the covalent binding reaction between said full length heparin and the amino groups is performed at 60°
C., andd) recovering the solid substrate having said full length heparin covalently bound on its surface.
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Abstract
The present invention relates to a device for extracorporeal removal of harmful agents from blood or blood components, comprising full length heparin immobilized on a solid substrate by covalent end point attachment. The present invention also relates to a method for extracorporeal removal of a harmful agent from mammalian blood or blood components. The present invention further relates to a process for covalent end point attachment of full length heparin to a solid substrate.
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Citations
32 Claims
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1. A device for extracorporeal removal of a harmful agent having a binding affinity to heparin from blood or blood components, the device comprising a full length heparin immobilized on a solid substrate by a covalent end point attachment, where said covalent end point attachment is formed by a process comprising the steps of:
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a) providing said solid substrate having primary amino functional groups, b) mixing said solid substrate of a) with said full length heparin and a reducing agent in an aqueous medium, c) allowing said full length heparin to bind covalently to the amino functional groups, wherein the covalent binding reaction between said full length heparin and the amino groups is performed at 60°
C., andd) recovering the solid substrate having said full length heparin covalently bound on its surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
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28. A process for forming a covalent end point attachment of a full length heparin to a solid substrate comprising the steps of:
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a) providing a solid substrate having primary amino functional groups, b) mixing said solid substrate of a) with said full length heparin and a reducing agent in an aqueous medium, c) allowing said full length heparin to bind covalently to the amino functional groups, wherein the covalent binding reaction between said full length heparin and the amino groups is performed at 60°
C., andd) recovering the solid substrate having said full length heparin covalently bound on its surface. - View Dependent Claims (29, 30, 31, 32)
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Specification