Method and apparatus for automatic staining of tissue samples

• US 8,663,978 B2
• Filed: 08/06/2012
• Issued: 03/04/2014
• Est. Priority Date: 12/20/2002
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Dako Denmark A/S (Agilent Technologies, Inc.)
• Inventors: Sweet, Doug, Key, Marc, Feingold, Gordon, Buchanan, Kristopher, Lathrop, Bob, Favuzzi, John
• Primary Examiner(s): Beisner, William H
• Assistant Examiner(s): HENKEL, DANIELLE B
• Application Number:
  US13/567,308
• Publication Number:
  US 20130029409A1
• Time in Patent Office: 575 Days
• Field of Search: 435/288.7, 435/287.3, 435/286.4, 422/60, 422/63, 422/64, 422/65
• US Class Current: 435/288.7
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00089 Magazines
• CPC Class Codes: G01N 2035/00306 Housings, cabinets, control panels (details)
• CPC Class Codes: G01N 2035/00326 Analysers with modular structure
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 2035/00376 Conductive heating, e.g. heated plates
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 2035/00881 network configurations
• CPC Class Codes: G01N 2035/00891 Displaying information to the operator
• CPC Class Codes: G01N 2035/009 alarms, e.g. audible
• CPC Class Codes: G01N 2035/0091 GUI [graphical user interfaces]
• CPC Class Codes: G01N 35/00712 Automatic status testing, e.g. at start-up or periodic
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: G01N 35/00871 Communications between instruments or with remote terminals
• CPC Class Codes: G01N 35/0092 Scheduling
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G16H 50/20 for computer-aided diagnosis, e.g. based on medical expert systems
• CPC Class Codes: Y02A 90/10 : Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
• CPC Class Codes: Y10S 901/02 : Arm motion controller
• CPC Class Codes: Y10S 901/41 : Tool
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/112499 : with sample on test slide
• CPC Class Codes: Y10T 436/113332 : with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114165 : with step of insertion or removal from test line
• CPC Class Codes: Y10T 436/114998 : with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

The present invention concerns an apparatus for staining tissue samples, said apparatus including a reagent section or reagent containers; at least one staining section or tissue samples, a robotic head or robotic element that may move reagent to a predetermined tissue sample, said robotic element being moveable above the reagent and the staining sections, a control element that may manage a staining process, a 2-D optical sensor to detect two-dimensional image data of a relevant property and that can feed the captured image data to the control element. By providing the robotic element with a 2-D optical sensor, a common image processor may be provided having multiple functions. By using a 2-D optical image processing system, the control system of the apparatus may easily be adapted to read various types of data presentations, just as actual images for sections of the apparatus may be identified in order to assess the condition of the apparatus. The optical sensor may be used to automatically identify the slides and the reagent containers present in the apparatus, just as the optical sensor may be used for checking if a slide is misplaced at or absent from a slide position, etc.

Claims

1. An automatic staining apparatus comprising:

at least one removable reagent container positioned on a reagent rack within a reagent section;

at least one slide positioned within a slide section;

a robotic element configured to move above the reagent section and above the slide section during a staining process, wherein the reagent rack is removable below the plane of the robotic element and wherein the reagent section is situated to enable the at least one removable reagent container to be added to or removed from the apparatus without interrupting the movement of the robotic element during dispensing of at least one reagent during the staining process;

an optical sensor associated with the robotic element; and

a control element, the control element configured to establish communication with a server;

direct operation of the robotic element;

direct operation of the optical sensor to obtain data;

communicate the data obtained by the optical sensor to the server; and

continue movement of the robotic element and dispensing of at least one reagent during insertion or removal of the at least one removable reagent container.

2. The automatic staining apparatus of claim 1, wherein the control element is adapted to locate, based on the data obtained by the optical sensor, pre-selected reference features for self-calibration of the robotic element.

3. The automatic staining apparatus of claim 1, wherein a sample is located on the at least one slide.

4. The automatic staining apparatus of claim 3, wherein the data obtained by the optical sensor includes an image of the finalized sample after said sample has been subjected to the staining process.

5. The automatic staining apparatus of claim 3, wherein the data obtained by the optical sensor includes an image of the sample before the sample has been subjected to the staining process.

6. The automatic staining apparatus of claim 1, further comprising:

at least one identification element provided on at least one of the at least one removable reagent container and the at least one slide;

wherein the at least one identification element comprises an identification element selected from a group consisting of;

a two-dimensional high-resolution symbology code, a datamatrix code, a bar code, an adhesive label, a two dimensional symbology zone, and a human readable text zone.

7. The automatic staining apparatus of claim 3, wherein the control element is configured to identify, based on the data obtained by the optical sensor, a feature selected from a group consisting of:

a texture of the sample, an outline of the sample, a visual property of the sample, and an individual identification feature of the sample.

8. The automatic staining apparatus of claim 1, wherein the control element is configured to automatically identify, based on the data obtained by the optical sensor, new slides and reagent bottles loaded into the apparatus during the staining process.

9. The automatic staining apparatus of claim 1, wherein the control element is configured to communicate the data obtained by the optical sensor to a central database on the server.

10. The automatic staining apparatus of claim 3, wherein the data obtained by the optical sensor is a sample image suitable for diagnostic purposes.

11. The automatic staining apparatus of claim 3, wherein the control element is further configured to determine a location of the sample on the slide based on data obtained by the optical sensor.

12. The automatic staining apparatus of claim 3, wherein the control element is further configured to adjust the staining process based on based on data obtained by the optical sensor.

13. The automatic staining apparatus of claim 1, wherein the optical sensor comprises a camera.

14. The automatic staining apparatus of claim 1, wherein the optical sensor comprises a CCD element.

15. An automatic staining apparatus comprising:

at least one removable reagent container within a reagent section;

at least one slide positioned on a slide rack within a slide section;

a robotic element configured to move above the reagent section and above the slide section during a staining process, wherein the slide rack is removable below the plane of the robotic element and wherein the slide section is situated to enable the at least one slide to be added to or removed from the apparatus without interrupting the

movement of the robotic element during dispensing of at least one reagent during the staining process;

an optical sensor associated with the robotic element; and

a control element, the control element configured to establish communication with a server;

direct operation of the robotic element;

direct operation of the optical sensor;

communicate data obtained by the optical sensor to the server; and

continue movement of the robotic element and dispensing of at least one reagent during insertion or removal of the at least one slide.

16. An automatic staining apparatus comprising:

at least one removable reagent container positioned on a reagent rack within a reagent section;

at least one slide positioned on a slide rack within a slide section;

a robotic element configured to move above the reagent section and above the slide section during a staining process, wherein the reagent rack is removable below the plane of the robotic element and wherein the reagent section and the slide section are situated to enable the at least one removable reagent container and the at least one slide to be added to or removed from the apparatus without interrupting the movement of the robotic element during dispensing of at least one reagent during the staining process;

an optical sensor associated with the robotic element; and

a control element, the control element configured to establish communication with a server;

direct operation of the robotic element;

direct operation of the optical sensor;

communicate data obtained by the optical sensor to the server; and

continue movement of the robotic element and dispensing of at least one reagent during insertion or removal of the at least one removable reagent container and the at least one slide.