IL-I binding proteins

• US 8,664,367 B2
• Filed: 03/20/2013
• Issued: 03/04/2014
• Est. Priority Date: 05/14/2010
• Status: Active Grant

• Current Assignee: Abbvie Incorporated
• Original Assignee: Abbvie Incorporated
• Inventors: Wu, Chengbin, Ambrosi, Dominic J., Hsieh, Chung-ming, Ghayur, Tariq
• Primary Examiner(s): DUFFY, BRADLEY
• Application Number:
  US13/847,862
• Publication Number:
  US 20130195754A1
• Time in Patent Office: 349 Days
• Field of Search: None
• US Class Current: 530/387.3
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/3955 against proteinaceous materials, e.g. enzymes, hormones, lymphokines
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61K 47/6879 the immunoglobulin having two or more different antigen-binding sites, e.g. bispecific or multispecific immunoglobulin
• CPC Class Codes: A61K 9/0014 Skin, i.e. galenical aspects of topical compositions non-active ingredients are additionally classified in A61K47/00; A61K9/0009, A61K9/0021, A61K9/7015, A61K9/7023 take precedence; cosmetic preparations A61K8/00, A61Q; preparations for wound dressings or bandages A61L26/00
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61K 9/0031 Rectum, anus
• CPC Class Codes: A61K 9/0034 Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
• CPC Class Codes: A61K 9/0043 Nose
• CPC Class Codes: A61K 9/0053 Mouth and digestive tract, i.e. intraoral and peroral administration rectal administration A61K9/0031
• CPC Class Codes: A61K 9/006 Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
• CPC Class Codes: A61K 9/007 Pulmonary tract; Aromatherapy
• CPC Class Codes: A61K 9/0085 Brain, e.g. brain implants; Spinal cord
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 1/14 Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 1/18 for pancreatic disorders, e.g. pancreatic enzymes
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 11/02 Nasal agents, e.g. decongestants
• CPC Class Codes: A61P 11/04 : for throat disorders
• CPC Class Codes: A61P 11/06 : Antiasthmatics
• CPC Class Codes: A61P 11/16 : Central respiratory analeptics
• CPC Class Codes: A61P 13/02 : of urine or of the urinary tract, e.g. urine acidifiers
• CPC Class Codes: A61P 13/08 : of the prostate
• CPC Class Codes: A61P 13/12 : of the kidneys
• CPC Class Codes: A61P 15/02 : for disorders of the vagina
• CPC Class Codes: A61P 15/08 : for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
• CPC Class Codes: A61P 15/10 : for impotence
• CPC Class Codes: A61P 17/00 : Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 : for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/04 : Antipruritics
• CPC Class Codes: A61P 17/06 : Antipsoriatics
• CPC Class Codes: A61P 17/08 : Antiseborrheics
• CPC Class Codes: A61P 17/14 : for baldness or alopecia
• CPC Class Codes: A61P 19/00 : Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 : for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/06 : Antigout agents, e.g. antihyperuricemic or uricosuric agents
• CPC Class Codes: A61P 19/10 : for osteoporosis
• CPC Class Codes: A61P 21/00 : Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 21/02 : Muscle relaxants, e.g. for tetanus or cramps
• CPC Class Codes: A61P 21/04 : for myasthenia gravis
• CPC Class Codes: A61P 25/00 : Drugs for disorders of the nervous system
• CPC Class Codes: A61P 25/04 : Centrally acting analgesics, e.g. opioids
• CPC Class Codes: A61P 25/06 : Antimigraine agents
• CPC Class Codes: A61P 25/14 : for treating abnormal movements, e.g. chorea, dyskinesia
• CPC Class Codes: A61P 25/16 : Anti-Parkinson drugs
• CPC Class Codes: A61P 25/18 : Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
• CPC Class Codes: A61P 25/22 : Anxiolytics
• CPC Class Codes: A61P 25/24 : Antidepressants
• CPC Class Codes: A61P 25/28 : for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 25/30 : for treating abuse or dependence
• CPC Class Codes: A61P 25/32 : Alcohol-abuse
• CPC Class Codes: A61P 27/02 : Ophthalmic agents
• CPC Class Codes: A61P 27/14 : Decongestants or antiallergics
• CPC Class Codes: A61P 29/00 : Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/00 : Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/02 : Nutrients, e.g. vitamins, minerals
• CPC Class Codes: A61P 3/06 : Antihyperlipidemics
• CPC Class Codes: A61P 3/08 : for glucose homeostasis pancreatic hormones A61P5/48
• CPC Class Codes: A61P 3/10 : for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 3/14 : for calcium homeostasis vitamin D A61P3/02; parathyroid hormones A61P5/18; calcitonin A61P5/22; osteoporosis A61P19/10; bone metastasis A61P35/04
• CPC Class Codes: A61P 31/00 : Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 31/04 : Antibacterial agents
• CPC Class Codes: A61P 31/06 : for tuberculosis
• CPC Class Codes: A61P 31/08 : for leprosy
• CPC Class Codes: A61P 31/10 : Antimycotics
• CPC Class Codes: A61P 31/12 : Antivirals
• CPC Class Codes: A61P 31/14 : for RNA viruses
• CPC Class Codes: A61P 31/16 : for influenza or rhinoviruses
• CPC Class Codes: A61P 31/18 : for HIV
• CPC Class Codes: A61P 31/20 : for DNA viruses
• CPC Class Codes: A61P 33/00 : Antiparasitic agents
• CPC Class Codes: A61P 33/02 : Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
• CPC Class Codes: A61P 33/06 : Antimalarials
• CPC Class Codes: A61P 33/08 : for Pneumocystis carinii
• CPC Class Codes: A61P 35/00 : Antineoplastic agents
• CPC Class Codes: A61P 35/02 : specific for leukemia
• CPC Class Codes: A61P 37/00 : Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/02 : Immunomodulators
• CPC Class Codes: A61P 37/04 : Immunostimulants
• CPC Class Codes: A61P 37/06 : Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 37/08 : Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: A61P 39/02 : Antidotes
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 5/00 : Drugs for disorders of the endocrine system
• CPC Class Codes: A61P 5/14 : of the thyroid hormones, e.g. T3, T4
• CPC Class Codes: A61P 5/16 : for decreasing, blocking or antagonising the activity of the thyroid hormones
• CPC Class Codes: A61P 5/18 : of the parathyroid hormones
• CPC Class Codes: A61P 5/48 : of the pancreatic hormones
• CPC Class Codes: A61P 5/50 : for increasing or potentiating the activity of insulin
• CPC Class Codes: A61P 7/02 : Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
• CPC Class Codes: A61P 7/04 : Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
• CPC Class Codes: A61P 7/06 : Antianaemics
• CPC Class Codes: A61P 7/08 : Plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for electrolytic or acid-base disorders, e.g. hypovolemic shock artificial tears A61P27/04
• CPC Class Codes: A61P 7/10 : Antioedematous agents; Diuretics
• CPC Class Codes: A61P 9/00 : Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/04 : Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
• CPC Class Codes: A61P 9/06 : Antiarrhythmics
• CPC Class Codes: A61P 9/08 : Vasodilators for multiple indications
• CPC Class Codes: A61P 9/10 : for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: A61P 9/12 : Antihypertensives
• CPC Class Codes: A61P 9/14 : Vasoprotectives; Antihaemorrhoidals; Drugs for varicose therapy; Capillary stabilisers
• CPC Class Codes: C07K 14/545 : IL-1
• CPC Class Codes: C07K 16/245 : IL-1
• CPC Class Codes: C07K 16/468 : Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
• CPC Class Codes: C07K 2317/24 : containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/31 : multispecific
• CPC Class Codes: C07K 2317/33 : Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
• CPC Class Codes: C07K 2317/56 : variable (Fv) region, i.e. VH and/or VL
• CPC Class Codes: C07K 2317/565 : Complementarity determining region [CDR]
• CPC Class Codes: C07K 2317/64 : comprising a combination of variable region and constant region components
• CPC Class Codes: C07K 2317/76 : Antagonist effect on antigen, e.g. neutralization or inhibition of binding
• CPC Class Codes: C07K 2317/92 : Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
• CPC Class Codes: G01N 2333/545 : IL-1
• CPC Class Codes: G01N 2800/00 : Detection or diagnosis of diseases
• CPC Class Codes: G01N 33/6869 : Interleukin
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

Proteins that bind IL-1α and IL-1β are described along with their use in compositions and methods for treating, preventing, and diagnosing IL-1-related disorders and for detecting IL-1α and IL-1β in cells, tissues, samples, and compositions.

Claims

1. A binding protein comprising first and second polypeptide chains, wherein said first polypeptide chain comprises a first VD1-(X1)n-VD2-C-(X2)n, wherein:

VD1 is a first heavy chain variable domain;

VD2 is a second heavy chain variable domain;

C is a heavy chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 is an Fc region; and

n is independently 0 or 1; and

wherein said second polypeptide chain comprises a second VD1-(X1)n-VD2-C-(X2)n, wherein;

VD1 is a first light chain variable domain;

VD2 is a second light chain variable domain;

C is a light chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 does not comprise an Fc region; and

n is independently 0 or 1;

wherein, in said first polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

204; and

VD2 comprises the amino acid sequence of SEQ ID NO;

213;

wherein, in said second polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

238; and

VD2 comprises the amino acid sequence of SEQ ID NO;

216; and

wherein the binding protein binds human IL-1β and

human IL-1α

.

3. The binding protein according to claim 1, wherein said first polypeptide chain comprises the amino acid sequence of SEQ ID NO:

212.

4. The binding protein according to claim 1, wherein said second polypeptide chain comprises the amino acid sequence of SEQ ID NO:

215.

7. The binding protein according to claim 1, wherein said first polypeptide chain comprises the amino acid sequence of SEQ ID NO:

212, and said second polypeptide chain comprises the amino acid sequence of SEQ ID NO;

215.

9. The binding protein according to claim 1, wherein the binding protein comprises two first polypeptide chains and two second polypeptide chains.

10. The binding protein according to claim 1, wherein X1 or X2 is an amino acid sequence selected from the group consisting of SEQ ID NOs:

26-57, 223, 224, and 225.

11. The binding protein according to claim 1, wherein the Fc region is a variant sequence Fc region.

12. A binding protein conjugate comprising a binding protein as described in claim 1, said binding protein conjugate further comprising an agent selected from the group consisting of:

an immunoadhesion molecule, an imaging agent, a therapeutic agent, and a cytotoxic agent.

13. The binding protein conjugate according to claim 12, wherein said agent is a therapeutic or cytotoxic agent selected from the group consisting of:

an anti-metabolite, an alkylating agent, an antibiotic, a growth factor, a cytokine, an anti-angiogenic agent, an anti-mitotic agent, an anthracycline, a toxin, and an apoptotic agent.

14. A pharmaceutical composition comprising a binding protein as described in claim 1, and a pharmaceutically acceptable carrier.

15. The pharmaceutical composition according to claim 14, further comprising at least one additional agent.

16. The pharmaceutical composition according to claim 15, wherein said additional agent is selected from the group consisting of:

a therapeutic agent;

an imaging agent;

a cytotoxic agent;

an angiogenesis inhibitor;

a kinase inhibitor;

a co-stimulation molecule blocker;

an adhesion molecule blocker;

an anti-cytokine antibody or functional fragment thereof;

methotrexate;

cyclosporin;

rapamycin;

FK506;

a detectable label or reporter;

a TNF antagonist;

an antirheumatic;

a muscle relaxant;

a narcotic;

a non-steroid anti-inflammatory drug (NSAID);

an analgesic;

an anesthetic;

a sedative;

a local anesthetic;

a neuromuscular blocker;

an antimicrobial;

an antipsoriatic;

a corticosteroid;

an anabolic steroid;

an erythropoietin;

an immunization;

an immunoglobulin;

an immunosuppressive;

a growth hormone;

a hormone replacement drug;

a radiopharmaceutical;

an antidepressant;

an antipsychotic;

a stimulant;

an asthma medication;

a beta agonist;

an inhaled steroid;

an epinephrine or analog thereof;

a cytokine; and

a cytokine antagonist.

17. A pharmaceutical composition comprising a binding protein conjugate as described in claim 12, and a pharmaceutically acceptable carrier.

18. The pharmaceutical composition according to claim 17, wherein said binding protein conjugate comprises a therapeutic or cytotoxic agent selected from the group consisting of:

an anti-metabolite, an alkylating agent, an antibiotic, a growth factor, a cytokine, an anti-angiogenic agent, an anti-mitotic agent, an anthracycline, a toxin, and an apoptotic agent.

2. A binding protein comprising first and second polypeptide chains, wherein said first polypeptide chain comprises a first VD1-(X1)n-VD2-C-(X2)n, wherein:

VD1 is a first heavy chain variable domain;

VD2 is a second heavy chain variable domain;

C is a heavy chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 is an Fc region; and

n is independently 0 or 1; and

wherein said second polypeptide chain comprises a second VD1-(X1)n-VD2-C-(X2)n, wherein;

VD1 is a first light chain variable domain;

VD2 is a second light chain variable domain;

C is a light chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 does not comprise an Fc region; and

n is independently 0 or 1;

wherein, in said first polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

213; and

VD2 comprises the amino acid sequence of SEQ ID NO;

204;

wherein, in said second polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

216; and

VD2 comprises the amino acid sequence of SEQ ID NO;

238; and

wherein the binding protein binds human IL-1β and

human IL-1α

.

5. The binding protein according to claim 2, wherein said first polypeptide chain comprises the amino acid sequence of SEQ ID NO:

219.

6. The binding protein according to claim 2, wherein said second polypeptide chain comprises the amino acid sequence of SEQ ID NO:

220.

8. The binding protein according to claim 2, wherein said first polypeptide chain comprises the amino acid sequence of SEQ ID NO:

219, and said second polypeptide chain comprises the amino acid sequence of SEQ ID NO;

220.

19. The binding protein according to claim 2, wherein the binding protein comprises two first polypeptide chains and two second polypeptide chains.

20. The binding protein according to claim 2, wherein X1 or X2 is an amino acid sequence selected from the group consisting of SEQ ID NOs:

26-57, 223, 224, and 225.

21. The binding protein according to claim 2, wherein the Fc region is a variant sequence Fc region.

22. A binding protein conjugate comprising a binding protein as described in claim 2, said binding protein conjugate further comprising an agent selected from the group consisting of:

an immunoadhesion molecule, an imaging agent, a therapeutic agent, and a cytotoxic agent.

23. The binding protein conjugate according to claim 22, wherein said agent is a therapeutic or cytotoxic agent selected from the group consisting of:

an anti-metabolite, an alkylating agent, an antibiotic, a growth factor, a cytokine, an anti-angiogenic agent, an anti-mitotic agent, an anthracycline, a toxin, and an apoptotic agent.

24. A pharmaceutical composition comprising a binding protein as described in claim 2, and a pharmaceutically acceptable carrier.

25. The pharmaceutical composition according to claim 24, further comprising at least one additional agent.

26. The pharmaceutical composition according to claim 25, wherein said additional agent is selected from the group consisting of:

a therapeutic agent;

an imaging agent;

a cytotoxic agent;

an angiogenesis inhibitor;

a kinase inhibitor;

a co-stimulation molecule blocker;

an adhesion molecule blocker;

an anti-cytokine antibody or functional fragment thereof;

methotrexate;

cyclosporin;

rapamycin;

FK506;

a detectable label or reporter;

a TNF antagonist;

an antirheumatic;

a muscle relaxant;

a narcotic;

a non-steroid anti- inflammatory drug (NSAID);

an analgesic;

an anesthetic;

a sedative;

a local anesthetic;

a neuromuscular blocker;

an antimicrobial;

an antipsoriatic;

a corticosteroid;

an anabolic steroid;

an erythropoietin;

an immunization;

an immunoglobulin;

an immunosuppressive;

a growth hormone;

a hormone replacement drug;

a radiopharmaceutical;

an antidepressant;

an antipsychotic;

a stimulant;

an asthma medication;

a beta agonist;

an inhaled steroid;

an epinephrine or analog thereof;

a cytokine; and

a cytokine antagonist.

27. A pharmaceutical composition comprising a binding protein conjugate as described in claim 22, and a pharmaceutically acceptable carrier.

28. The pharmaceutical composition according to claim 27, wherein said agent is a therapeutic or cytotoxic agent selected from the group consisting of:

an anti-metabolite, an alkylating agent, an antibiotic, a growth factor, a cytokine, an anti-angiogenic agent, an anti-mitotic agent, an anthracycline, a toxin, and an apoptotic agent.

29. A binding protein comprising first and second polypeptide chains, wherein said first polypeptide chain comprises a first VD1-(X1)n-VD2-C-(X2)n, wherein:

VD1 is a first heavy chain variable domain;

VD2 is a second heavy chain variable domain;

C is a heavy chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 is an Fc region; and

n is independently 0 or 1; and

wherein said second polypeptide chain comprises a second VD1-(X1)n-VD2-C-(X2)n, wherein;

VD1 is a first light chain variable domain;

VD2 is a second light chain variable domain;

C is a light chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 does not comprise an Fc region; and

n is independently 0 or 1;

wherein, in said first polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

204; and

VD2 comprises the amino acid sequence of SEQ ID NO;

213;

wherein, in said second polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

238; and

VD2 comprises the amino acid sequence of SEQ ID NO;

216; and

wherein said first polypeptide chain comprises the amino acid sequence of SEQ ID NO;

212, and

said second polypeptide chain comprises the amino acid sequence of SEQ ID NO;

215; and

wherein the binding protein binds human IL-1β and

human IL-1α

.

30. A binding protein comprising first and second polypeptide chains, wherein said first polypeptide chain comprises a first VD1-(X1)n-VD2-C-(X2)n, wherein:

VD1 is a first heavy chain variable domain;

VD2 is a second heavy chain variable domain;

C is a heavy chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 is an Fc region; and

n is independently 0 or 1; and

wherein said second polypeptide chain comprises a second VD1-(X1)n-VD2-C-(X2)n, wherein;

VD1 is a first light chain variable domain;

VD2 is a second light chain variable domain;

C is a light chain constant domain;

X1 is a linker with the proviso that it is not CH1;

X2 does not comprise an Fc region; and

n is independently 0 or 1;

wherein, in said first polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

213; and

VD2 comprises the amino acid sequence of SEQ ID NO;

204;

wherein, in said second polypeptide chain,

VD1 comprises the amino acid sequence of SEQ ID NO;

216; and

VD2 comprises the amino acid sequence of SEQ ID NO;

238; and

wherein said first polypeptide chain comprises the amino acid sequence of SEQ ID NO;

219, and

said second polypeptide chain comprises the amino acid sequence of SEQ ID NO;

220; and

wherein the binding protein binds human IL-1β and

human IL-1α

.