Lyotropic liquid crystal coated analyte monitoring device and methods of use
First Claim
1. A method of monitoring the concentration of an analyte, the method comprising:
- positioning at least a portion of an electrochemical sensor beneath the surface of the skin of a subject, wherein the electrochemical sensor comprises;
a working electrode comprising a sensing layer comprising an analyte-responsive enzyme and a redox mediator in contact with the electrode and a non-crosslinked cubic phase lyotropic liquid crystal membrane disposed on top of the sensing layer; and
a counter electrode or reference electrode or a counter/reference electrode in communication with the working electrode;
applying a potential between the working electrode and the counter electrode to electrolyze the analyte and measuring a current from the applied potential; and
determining the concentration of the analyte from the measured current.
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Abstract
The present invention is directed to membranes composed liquid crystals having continuous aqueous channels, such as a lyotropic liquid crystal, including a cubic phase lyotropic liquid crystal, and to electrochemical sensors equipped with such membranes. The membranes are useful in limiting the diffusion of an analyte to a working electrode in an electrochemical sensor so that the sensor does not saturate and/or remains linearly responsive over a large range of analyte concentrations. Electrochemical sensors equipped with membranes of the present invention demonstrate considerable sensitivity and stability, and a large signal-to-noise ratio, in a variety of conditions.
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Citations
15 Claims
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1. A method of monitoring the concentration of an analyte, the method comprising:
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positioning at least a portion of an electrochemical sensor beneath the surface of the skin of a subject, wherein the electrochemical sensor comprises; a working electrode comprising a sensing layer comprising an analyte-responsive enzyme and a redox mediator in contact with the electrode and a non-crosslinked cubic phase lyotropic liquid crystal membrane disposed on top of the sensing layer; and a counter electrode or reference electrode or a counter/reference electrode in communication with the working electrode; applying a potential between the working electrode and the counter electrode to electrolyze the analyte and measuring a current from the applied potential; and determining the concentration of the analyte from the measured current. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification