Methods for genetic analysis of textiles made of Gossypium barbadense and Gossypium hirsutum cotton

• US 8,669,079 B2
• Filed: 11/12/2008
• Issued: 03/11/2014
• Est. Priority Date: 11/12/2008
• Status: Active Grant

• Current Assignee: APDN (B.V.I.) Inc. (Applied DNA Sciences Incorporated)
• Original Assignee: Cara Therapeutics, Inc.
• Inventors: Liang, Ming-Hwa, Shu Kin So, Stephane
• Primary Examiner(s): Pande, Suchira
• Application Number:
  US12/269,737
• Publication Number:
  US 20110250594A1
• Time in Patent Office: 1,945 Days
• Field of Search: None
• US Class Current: 435/91.2
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

Methods for distinguishing between cotton species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the species of cotton utilized in the textile or cotton material of interest.

Claims

1. A method for identifying cotton species comprising:

collecting one or more mature cotton fibers from a sample;

extracting genomic DNA from the mature cotton fibers collected, wherein the genomic DNA extracted from the mature cotton fibers comprises chloroplast DNA;

amplifying said chloroplast DNA with a PCR-based technique producing one or more amplified products; and

analyzing the one or more amplified products to distinguish between a first cotton species and a second cotton species by detecting a length polymorphism.

2. The method of claim 1, wherein one of the first cotton species and the second cotton species is G. barbadense.

3. The method of claim 1, wherein one of the first cotton species and the second cotton species is G. hirsutum.

4. The method of claim 1, wherein the sample is selected from a group consisting of raw mature cotton, ginned mature cotton, yarn blended with mature cotton, and fabric blended with mature cotton.

5. The method of claim 1, wherein the amplifying further comprises using two or more sets of specific primers which target a length polymorphism between the first cotton species and the second cotton species.

6. The method of claim 3, wherein the amplifying further comprises using at least one set of specific primers targeting polymorphic sequences differing in length between G. barbadense and G. hirsutum.

7. The method of claim 6, wherein the polymorphic sequences of the first cotton species and the second cotton species targeted by the specific primers differ in an additional detectable physical characteristic in addition to the difference in sequence length.

8. The method of claim 7, wherein the additional detectable physical characteristic comprises a difference in nucleotide composition.

9. The method of claim 6, wherein said analyzing the one or more amplified products further comprises the use of a hybridizing probe specific for a target sequence of either G. barbadense or G. hirsutum.

10. The method of claim 1, wherein the analyzing of the one or more amplified products comprises detecting the size of the one or more amplified products by gel electrophoresis.

11. The method of claim 1, wherein the analyzing of the one or more amplified products comprises detecting the size of the one or more amplified products by capillary electrophoresis.

12. The method of claim 1, wherein the analyzing of the one or more amplified products comprises detecting the size of the one or more amplified products amplified by real-time PCR.

13. The method of claim 1, where the one of the first cotton species and the second cotton species is G. arboreum.

14. The method of claim 1, where the one of the first cotton species and the second cotton species is G. herbaceum.

15. The method of claim 1 wherein said sample is obtained from a textile.

16. The method of claim 12, further comprising quantifying one or more of the amplified products by real-time PCR.

17. The method of claim 2, wherein the other of the first cotton species and the second cotton species is G. hirsutum.