Bio-microarray and a substrate for use therewith

• US 8,669,098 B2
• Filed: 10/06/2010
• Issued: 03/11/2014
• Est. Priority Date: 03/14/2003
• Status: Expired due to Fees

• Current Assignee: Dai Nippon Printing Company Limited
• Original Assignee: Dai Nippon Printing Company Limited
• Inventors: Hattori, Hideshi
• Primary Examiner(s): LAM, ANN Y
• Application Number:
  US12/899,007
• Publication Number:
  US 20110021380A1
• Time in Patent Office: 1,252 Days
• Field of Search: None
• US Class Current: 435/287.9
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Abstract

For the purpose of improving the accuracy of a variety analyses using a bio-microarray, it is a main object of the invention to provide a bio-microarray capable of producing relatively high signal intensity from a fluorescent molecule and capable of having improved quantification performance and to provide a substrate for bio-microarray.

In order to achieve the object, the invention provides a substrate for bio-microarray characterized by having a reflection-suppressing function.

Claims

1. A substrate for bio-microarray comprising:

a substrate;

an anti-reflection layer formed on the surface of the substrate; and

an immobilization layer for immobilizing a probe biomolecule formed in a pattern on the surface of the substrate, wherein the anti-reflection layer is formed only in a region other than a region in which the immobilization layer is formed.

2. The substrate for bio-microarray of claim 1, wherein the anti-reflection layer has a fine uneven structure comprising a fine particle of diameter in a range of 50 nm to 300 nm;

and

further wherein a bulk refractive index of the fine particle is smaller than that of the substrate.

3. The substrate for bio-microarray according to claim 1, wherein the anti-reflection layer has a fine uneven structure with a depth in a range of 80 nm to 250 nm;

and

further wherein a refractive index of the anti-reflection layer is smaller than that of the substrate.

4. The substrate for bio-microarray according to claim 1, wherein the anti-reflection layer has a fine porous structure;

and

further wherein a refractive index of the anti-reflection layer is smaller than that of the substrate.

5. The substrate for bio-microarray according to claim 1, further comprising a mark formed on the substrate for positional detection.

6. A bio-microarray comprising the substrate for bio-microarry of claim 1, and the probe biomolecule immobilized on the immobilization layer of the substrate.

7. The substrate for bio-microarray according to claim 1, further comprising a reflection layer between the substrate and the immobilization layer.