Automated instrumentation and method for measurements of samples

• US 8,669,112 B2
• Filed: 06/12/2008
• Issued: 03/11/2014
• Est. Priority Date: 06/12/2007
• Status: Active Grant

• Current Assignee: Wallac Oy (Revvity, Inc.)
• Original Assignee: Wallac Oy (Revvity, Inc.)
• Inventors: Heinonen, Aarne, Karunen, Juha, Korpi, Jarmo, Nurmi, Jarmo, Ojala, Markku, Salminen, Timo, White, Paul
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US12/664,331
• Publication Number:
  US 20100203573A1
• Time in Patent Office: 2,098 Days
• Field of Search: 422/63, 422/64
• US Class Current: 436/43
• CPC Class Codes: G01N 2001/288 Filter punches
• CPC Class Codes: G01N 2035/00108 Test strips, e.g. paper
• CPC Class Codes: G01N 2035/00326 Analysers with modular structure
• CPC Class Codes: G01N 35/0099 comprising robots or similar manipulators robots per se B25J
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

Instrumentation and a method for efficient and reliable assaying and measuring samples. The teachings include an automated self-contained instrument, wherein the samples are located on wells of sample plates, and the instrument includes a plurality of units for processing or storing sample plates. The instrument may includes at least one dispensing unit for dispensing reagents or other assay components to the sample wells, at least two units for simultaneously processing or storing a plurality of sample plates, at least one unit for removing substance from the sample wells, and one or several measurement units for optically measuring samples in at least two measurement modes. Further, the instrument includes a manipulator for moving the sample plates in three orthogonal directions or combinations thereof and for rotating the sample plates in relation to a vertical axis for transferring the sample plates to the units.

Claims

1. An automated, self-contained instrument for assaying and measuring of samples, wherein the samples are sample discs impregnated with blood and located in wells of sample plates, the instrument comprising:

(a) at least 4 vertical frames in adjacent pairs forming a frame structure;

(b) a turnable manipulator located inside the frame structure for transferring sample plates in three orthogonal directions or combinations thereof;

(c) a plurality of units for processing or storing sample plates, the units being installed on and outside the frame structure and adjacent to said turnable manipulator in order to be accessible by the turnable manipulator, said units comprising;

(i) at least one dispensing unit for dispensing reagents or other assay components to the sample wells,

(ii) at least two units for simultaneously processing or storing a plurality of sample plates,

(iii) at least one unit for removing substance from the sample wells, and

(iv) one or several measurement units, the measurement unit(s) providing a capability for the instrument to optically measure samples in at least two measurement modes,

wherein the turnable manipulator is configured to turn by at least 270 degrees in relation to a vertical axis in order to transfer sample plates between all units installed on the frame structure.

2. The instrument according to claim 1, wherein at least one unit for processing or storing sample plates is a module which is designed to allow installation and removal as a whole to/from the instrument.

3. The instrument according to claim 2, wherein the module comprises a front panel having a positioning means to position the module in relation to a location for a sample plate in the module, the front panel being arranged in a stationary position with respect to the turnable manipulator for positioning the module with respect to the frame structure.

4. The instrument according to claim 3, wherein the position of the front panel with respect to the turnable manipulator is adjustable at least in the horizontal plane for allowing exact mutual positioning of the front panel and the turnable manipulator.

5. The instrument according to claim 3, wherein the front panel is designed to significantly contribute to the rigidity of a structure supporting the turnable manipulator.

6. The instrument according to claim 3, wherein the turnable manipulator and the frame structure, and optionally the front panel(s), form an integral unit.

7. The instrument according to claim 1, wherein a module can be installed in alternative positions within the frame structure, whereby the information on the locations of the modules is stored in the system for controlling the movements of the turnable manipulator.

8. The instrument according to claim 1, further comprising at least one plate holder for receiving and holding a sample plate.

9. The instrument according to claim 1, wherein the at least two units for simultaneously processing or storing a plurality of sample plates comprise at least two incubator units which are arranged to provide simultaneous processing in different processing modes.

10. The instrument according to claim 1, wherein the at least one unit for removing substance from the sample wells is a washing unit for washing the wells of the sample plates.

11. The instrument according to claim 1, wherein the at least one unit for removing substance from the sample wells is a disc remover unit for removing a sample disc from a sample well.

12. The instrument according to claim 1, wherein the instrument comprises an air dryer for controlling the humidity of the air within the instrument and within a plate storage unit, wherein the air dryer comprises controlling means for controlling the humidity of the air into a humidity value that is adjustable within a determined range.

13. A method for assaying and measuring of blood samples in the automated self-contained instrument of claim 1, wherein the samples are impregnated into sample discs and located in wells of sample plates, and the method comprises phases in which a sample plate is processed in the plurality of processing units installed on the frame structure and the sample plate is moved between the units

for processing or storing by the turnable manipulator located inside the frame structure, the method comprising phases in which:

reagents or other assay components are dispensed to the sample wells,

a plurality of sample plates are simultaneously processed or stored in at least two units,

substance is removed from the sample wells,

samples are optically measured in at least two measurement modes, and

the sample plates are moved by the turnable manipulator in three orthogonal directions or combinations thereof including being rotated by the turnable manipulator by at 270 degrees inrelation to a vertical axis to transfer the sample plates between the processing units.

14. The method according to claim 13, wherein the at least one unit for processing or storing sample plates is installed or removed as a whole module to/from the instrument, the module being positioned on the instrument with positioning means, wherein the positioning means are accurately positioned in a front panel of the module in relation to the location for a sample plate in the module, thereby allowing a change of a module without individual calibration of the mechanical interface between the turnable manipulator and the module.