Biosensor

• US 8,673,127 B2
• Filed: 12/21/2010
• Issued: 03/18/2014
• Est. Priority Date: 03/29/2000
• Status: Expired due to Term

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Miyazaki, Shoji, Tokunaga, Hiroyuki, Fujiwara, Masaki, Nakaminami, Takahiro, Nakayama, Junko, Yamanishi, Eriko
• Primary Examiner(s): Noguerola, Alex
• Application Number:
  US12/928,841
• Publication Number:
  US 20110180404A1
• Time in Patent Office: 1,183 Days
• Field of Search: 20440301-40315, 435/817
• US Class Current: 204/403.14
• CPC Class Codes: C12Q 1/003 Functionalisation

Abstract

In a biosensor for measuring a specific substance in a liquid sample, one or a combination of sugar alcohol, metallic salt, organic acid or organic acid salt which has at least one carboxyl group in a molecule, and organic acid or organic acid salt which has at least one carboxyl group and one amino group in a molecule, is included in a reagent layer provided on electrodes, thereby providing a highly-accurate biosensor which is excellent in stability and has high response (sensitivity, linearity) of the sensor to the substrate concentration.

Claims

1. A biosensor for measuring the concentration of a specific substance in a sample solution, the biosensor comprising

electrodes comprising at least a working electrode and a counter electrode provided on an insulating support;

and

a reagent layer which reacts with the specific substance in the sample solution;

wherein the reagent layer comprises an enzyme, an electron transfer agent, and an organic acid or organic acid salt which has at least one carboxyl group;

the organic acid is aliphatic carboxylic acid, carbocyclic carboxylic acid, heterocyclic carboxylic acid, a substitution product, or derivative of the organic acid;

the carboxylic acid is malonic acid, succinic acid, glutaric acid, adipic acid, maleic acid, fumaric acid, benzoic acid, phthalic acid, isophthalic acid, terephthalic acid, 2-phthalic acid, nicotinic acid, isonicotinic acid, their salt, or a combination thereof; and

the enzyme is glucose dehydrogenase.

2. The biosensor as defined in claim 1, wherein the reagent layer comprises a hydrophilic polymer.

3. A biosensor for measuring the concentration of a specific substance in a sample solution, the biosensor comprising

electrodes comprising at least a working electrode and a counter electrode provided on an insulating support;

and

a reagent layer which reacts with the specific substance in the sample solution;

wherein the reagent layer includes an enzyme, an electron transfer agent, and an organic acid or organic acid salt which has at least one carboxyl group and one amino group;

the organic acid is an amino acid, a substitution product of an amino acid, sarcosine or betaine;

the amino acid is alanine, valine, leucine, isoleucine, serine, threonine, methionine, asparagine, glutamine, arginine, lysine, histidine, phenylalanine, tryptophan, proline, their salt, or a combination thereof; and

the enzyme is glucose dehydrogenase.

4. The biosensor as defined in claim 3, wherein the reagent layer comprises a hydrophilic polymer.