Bioanalytical instrumentation using a light source subsystem
First Claim
1. A system for use in analyzing one or more analyte comprising:
- one or more light pipes for use in generating luminescence, wherein each light pipe includesan excitation source which provides excitation energy, anda luminescent material which acts as a secondary absorber of the excitation energy, and wherein the luminescent material, when excited by the excitation source, generates and emits luminescence through an exit region of the light pipe;
relay optics which are used todirect the luminescence at one or more wavelengths, from the one or more light pipes, onto one or more analyte, anddirect emitted light, from the one or more analyte as a reaction to the luminescence, onto one or more light detector; and
wherein the emitted light is used to detect one or more of the presence or characteristics of the one or more analyte.
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Abstract
The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems. The capillaries are used to separate molecules that are chemically tagged with appropriate fluorescent or chromophore groups.
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Citations
24 Claims
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1. A system for use in analyzing one or more analyte comprising:
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one or more light pipes for use in generating luminescence, wherein each light pipe includes an excitation source which provides excitation energy, and a luminescent material which acts as a secondary absorber of the excitation energy, and wherein the luminescent material, when excited by the excitation source, generates and emits luminescence through an exit region of the light pipe; relay optics which are used to direct the luminescence at one or more wavelengths, from the one or more light pipes, onto one or more analyte, and direct emitted light, from the one or more analyte as a reaction to the luminescence, onto one or more light detector; and wherein the emitted light is used to detect one or more of the presence or characteristics of the one or more analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification