Microfluidic devices and methods

• US 8,673,240 B2
• Filed: 09/15/2008
• Issued: 03/18/2014
• Est. Priority Date: 02/23/2000
• Status: Expired due to Fees

• Current Assignee: Zyomyx, Inc.
• Original Assignee: Zyomyx, Inc.
• Inventors: Jedrzejewski, Paul, Wagner, Peter, Indermuhle, Pierre, Zaugg, Frank G., Steffen, Nock
• Primary Examiner(s): Sines, Brian J
• Application Number:
  US12/210,389
• Publication Number:
  US 20090028755A1
• Time in Patent Office: 2,010 Days
• Field of Search: 422/68.1, 422/81, 422/100, 422/103, 422/502, 422/503, 422/509, 436/43
• US Class Current: 422/503
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00367 capillary
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/00504 Pins
• CPC Class Codes: B01J 2219/00509 Microcolumns
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00587 High throughput processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00644 : the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00664 : Three-dimensional arrays
• CPC Class Codes: B01J 2219/00677 : Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00702 : Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B01L 2300/0809 : rectangular shaped
• CPC Class Codes: B01L 2300/0848 : Specific forms of parts of containers
• CPC Class Codes: B01L 2400/022 : droplet contacts the surface of the receptacle
• CPC Class Codes: B01L 2400/0688 : surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/0241 : Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: B01L 3/0244 : using pins
• CPC Class Codes: B01L 3/0248 : Prongs, quill pen type dispenser
• CPC Class Codes: B01L 3/0262 : using touch-off at substrate or container
• CPC Class Codes: B01L 3/5085 : for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/5088 : confining liquids at a location by surface tension, e.g. virtual wells on plates, wires B01L3/50857 takes precedence
• CPC Class Codes: B33Y 80/00 : Products made by additive manufacturing
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/114165 : with step of insertion or removal from test line
• CPC Class Codes: Y10T 436/117497 : with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/118339 : with formation of a segmented stream
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/156666 : Sulfur containing
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/25375 : Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 : including use of a solid sorbent, semipermeable membrane, or liquid extraction
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

Disclosed herein are devices and methods useful for the detection and analysis of various analytes. The analyte detection device of this invention has the general structure of a substrate and a plurality of pillars attached to the surface of the substrate.

Claims

1. An analyte detection device comprising a substrate and a plurality of pillars attached to the surface of the substrate, wherein each pillar comprises a sample surface and side surfaces, each of the side surfaces perpendicular to the surface of the substrate, and wherein the sample surface of each pillar and the surface of the substrate have the same coating, and wherein each pillar comprises a porous material.

2. The device of claim 1, wherein the side surfaces of each pillar, the sample surface of each pillar, and the surface of the substrate have the same coating.

3. The device of claim 1, further comprising an interlayer between the sample surfaces and the coating on the sample surfaces.

4. The device of claim 1, further comprising an interlayer between the substrate surface and the coating on the substrate surface.

5. The device of claim 3 or 4, wherein the interlayer comprises silicon oxide.

6. The device of claim 1 or 2, wherein the coating further comprises an affinity structure.

7. The device of claim 6, wherein the affinity structure comprises a monolayer of an organic molecule.

8. The device of claim 7, wherein the organic molecule is polylysine or polyethylene glycol.

9. The device of claim 6, wherein the affinity structure comprises a capture agent.

10. The device of claim 6, wherein the capture agent comprises an antibody, antibody fragment, polypeptide, receptor, DNA strand or fragment, RNA strand or fragment, or aptamer.

11. The device of claim 6, wherein the affinity structure comprises biotin, avidin, or streptavidin.

12. The device of claim 1, wherein the substrate and plurality of pillars are made of the same material.

13. The device of claim 1, wherein the substrate and plurality of pillars are made of different materials.

14. The device of claim 1, wherein the plurality of pillars are made of a porous material.

15. The device of claim 1, wherein the sample surface of each pillar is located distal to the substrate surface.

16. The device of claim 1, wherein the plurality of pillars are substantially perpendicular with respect to the surface of the substrate.

17. The device of claim 1, wherein the plurality of pillars range in height from about 1 to 200 microns.

18. The device of claim 1, wherein the plurality of pillars are arranged in a regular array.

19. The device of claim 1, wherein the plurality of pillars are arranged in an irregular array.

20. The device of claim 1, wherein the density of the plurality of pillars is about 25 pillars/cm² or greater.

21. The device of claim 1, wherein the number of pillars per device is greater than 10, 100, or 1000 pillars.

22. The device of claim 1, wherein the pillar pitch is 500 microns or less.

23. The device of claim 1, wherein the sample surface of each pillar or coating of each pillar binds an analyte.

24. The device of claim 1, wherein the analyte is selected from the group consisting of pharmaceuticals, metabolites, pesticides, polypeptides, proteins, DNA, and RNA.