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Method for isolation of afterbirth derived cells

  • US 8,673,547 B2
  • Filed: 03/07/2008
  • Issued: 03/18/2014
  • Est. Priority Date: 03/08/2007
  • Status: Active Grant
First Claim
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1. A method for extracting nucleated cells from afterbirth tissue, comprising the steps of:

  • a) locating the afterbirth tissue in a perfusion circuit without exsanguination of said afterbirth tissue;

    b) extracting nucleated cells from said non-exsanguinated afterbirth tissue by pulsatile perfusion carried out using a rhythmic, intermittent flow of a perfusion solution pulsed at between 50 and 60 pulses per minute to produce a perfusate containing cells; and

    c) isolating said nucleated cells from the perfusate.

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