Detecting neoplasm

• US 8,673,555 B2
• Filed: 02/11/2009
• Issued: 03/18/2014
• Est. Priority Date: 02/15/2008
• Status: Active Grant

• Current Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Original Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Inventors: Taylor, William R., Harrington, Jonathan J., Quint, Patrick S., Zou, Hongzhi, Bergen, Harold R. III, Smith, David I., Ahlquist, David A.
• Primary Examiner(s): AEDER, SEAN E
• Application Number:
  US12/866,558
• Publication Number:
  US 20110183328A1
• Time in Patent Office: 1,861 Days
• Field of Search: None
• US Class Current: 435/6.1
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: C12Q 1/40 involving amylase
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/154 Methylation markers
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: C12Y 302/01001 Alpha-amylase (3.2.1.1)
• CPC Class Codes: C12Y 304/2107 Pancreatic endopeptidase E (3.4.21.70)
• CPC Class Codes: G01N 2333/928 acting on alpha -1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
• CPC Class Codes: G01N 2333/966 Elastase
• CPC Class Codes: G01N 2560/00 Chemical aspects of mass spectrometric analysis of biological material
• CPC Class Codes: G01N 33/57407 Specifically defined cancers
• CPC Class Codes: G01N 33/57419 of colon
• CPC Class Codes: G01N 33/57438 of liver, pancreas or kidney

Abstract

This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.

Claims

1. A method of detecting colorectal cancer or pre-cancer in a mammal, said method comprising:

a) obtaining a stool sample from a mammal; and

b) measuring a K-ras mutation score within said stool sample, measuring BMP3 methylation status within said stool sample, and measuring the level of human DNA within said stool sample,

wherein measurement of an elevated K-ras mutation score, elevated BMP methylation status, and elevated human DNA level as compared to a normal control indicates that said mammal has said colorectal cancer or pre-cancer.

2. The method of claim 1, wherein said measuring of said K-ras mutation score is measured by digital melt curve analysis.

3. The method of claim 1, wherein said measuring of said K-ras mutation score is measured by quantitative allele-specific PCR.

4. A method of detecting aero-digestive cancer or pre-cancer in a mammal, said method comprising;

a) obtaining a stool sample from a mammal; and

b) measuring a K-ras mutation score within said stool sample, measuring BMP3 methylation status within said stool sample, and measuring the level of human DNA within said stool sample,

wherein measurement of an elevated K-ras mutation score, elevated BMP methylation status, and elevated human DNA level as compared to a normal control indicates that said mammal has said aero-digestive cancer or pre-cancer.

5. The method of claim 4, wherein said measuring of said K-ras mutation score is measured by digital melt curve analysis.

6. The method of claim 4, wherein said measuring of said K-ras mutation score is measured by quantitative allele-specific PCR.

7. The method of claim 4, wherein said method further comprises determining whether or not a stool sample from said mammal comprises an elevated APC mutation score.

8. The method of claim 7, wherein said APC mutation score can be measured by digital melt curve analysis.