Biosensor for analyzing quantitatively analyte with a predetermined size and larger than, and manufacturing method thereof
First Claim
1. A biosensor for quantitatively analyzing a material having a predetermined and larger size, the biosensor comprising:
- a) an electrode substrate;
b) a plurality of first monolayer molecules having electrochemical conductivity and forming an exposed conductive region on the electrode substrate, the exposed conductive region having a diameter less than that of the material to be analyzed; and
c) a plurality of second monolayer molecules formed on the electrode substrate excluding the area where the first monolayer molecules are formed, the second monolayer molecules having electrochemical dielectricity.
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Abstract
The present invention discloses a biosensor for quantitatively analyzing a bio-material and a manufacturing method thereof. The biosensor has an exposed conductive region of a few-nanometer scale distributed on an insulated metallic substrate in a desired pattern or randomly. The quantitative analysis of protein can be carried out by means of simplified procedures, without the necessity of rinsing out a signal-producing material, which is non-specifically bonded to the materials to be analyzed. The biosensor utilizes only the size of the molecules, and thus can be universally used for the analysis of bio-materials. A selective and separate analysis can be realized in which interference caused by other materials is significantly reduced.
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Citations
13 Claims
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1. A biosensor for quantitatively analyzing a material having a predetermined and larger size, the biosensor comprising:
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a) an electrode substrate; b) a plurality of first monolayer molecules having electrochemical conductivity and forming an exposed conductive region on the electrode substrate, the exposed conductive region having a diameter less than that of the material to be analyzed; and c) a plurality of second monolayer molecules formed on the electrode substrate excluding the area where the first monolayer molecules are formed, the second monolayer molecules having electrochemical dielectricity. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification