Biosensor for analyzing quantitatively analyte with a predetermined size and larger than, and manufacturing method thereof

• US 8,673,626 B2
• Filed: 02/07/2008
• Issued: 03/18/2014
• Est. Priority Date: 05/23/2003
• Status: Active Grant

• Current Assignee: ALL Medicus Co., Ltd.
• Original Assignee: ALL Medicus Co., Ltd.
• Inventors: Chang, Je-Young, Kang, Seung-Joo
• Primary Examiner(s): Chin, Chris L
• Application Number:
  US12/069,273
• Publication Number:
  US 20080311648A1
• Time in Patent Office: 2,231 Days
• Field of Search: None
• US Class Current: 435/287.2
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2610/00 Assays involving self-assembled monolayers [SAMs]
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 428/31507 Of polycarbonate

Abstract

The present invention discloses a biosensor for quantitatively analyzing a bio-material and a manufacturing method thereof. The biosensor has an exposed conductive region of a few-nanometer scale distributed on an insulated metallic substrate in a desired pattern or randomly. The quantitative analysis of protein can be carried out by means of simplified procedures, without the necessity of rinsing out a signal-producing material, which is non-specifically bonded to the materials to be analyzed. The biosensor utilizes only the size of the molecules, and thus can be universally used for the analysis of bio-materials. A selective and separate analysis can be realized in which interference caused by other materials is significantly reduced.

Claims

1. A biosensor for quantitatively analyzing a material having a predetermined and larger size, the biosensor comprising:

a) an electrode substrate;

b) a plurality of first monolayer molecules having electrochemical conductivity and forming an exposed conductive region on the electrode substrate, the exposed conductive region having a diameter less than that of the material to be analyzed; and

c) a plurality of second monolayer molecules formed on the electrode substrate excluding the area where the first monolayer molecules are formed, the second monolayer molecules having electrochemical dielectricity.

2. A biosensor according to claim 1, wherein the second monolayer molecules forms an insulated region.

3. A biosensor according to claim 1, wherein the exposed conductive region from the first monolayer molecules has a constant diameter or area.

4. A biosensor according to claim 1, wherein the exposed conductive region formed by the first monolayer molecules has a nano-scale diameter.

5. A biosensor according to claim 1, further comprising signal medium formed of a first material which can be bound specifically to the material to be analyzed and a second material which can produce an electro-chemical signal, the signal medium being able to diffuse through the exposed conductive region formed by the first monolayer molecules.

6. A biosensor according to claim 1, wherein the first and second monolayer molecules include a self-assembled monolayer molecules.

7. A biosensor according to claim 6, wherein the first monolayer molecules is formed of an organic compound containing thiols or disulfides.

8. A biosensor according to claim 6, the second monolayer molecules is formed of an organic compound containing thiols or disulfides,

9. A biosensor according to claim 7, wherein the first monolayer molecules is formed of a compound which is able to form a self-assembled monolayer molecules having a length that enables electrochemical current flow.

10. A biosensor according to claim 9, wherein the first monolayer molecules is formed of any one selected from the group consisting of 3-mercatopropionic acid, cysteamine, and cystamine.

11. A biosensor according to claim 1, wherein the second monolayer molecules is formed of a compound which is able to form a self-assembled monolayer molecules having a length that enables electrochemical insulation.

12. A biosensor according to claim 11, wherein the second monolayer molecules is formed of a 1-decanethiol series compound.

13. A biosensor according to claim 1, wherein the electrode substrate is formed of gold or platinum.