DNA hybrids and environment cleaning system employing DNA hybrids

• US 8,674,163 B2
• Filed: 01/16/2007
• Issued: 03/18/2014
• Est. Priority Date: 05/29/2003
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Zhang, Zuyi, Sakakibara, Teigo, Kotani, Yoshinori, Nishi, Norio
• Primary Examiner(s): Kim, Taeyoon
• Assistant Examiner(s): Patury, Srikanth
• Application Number:
  US11/653,233
• Publication Number:
  US 20070135672A1
• Time in Patent Office: 2,618 Days
• Field of Search: 588/315, 436/525, 436/527, 435/243
• US Class Current: 588/315
• CPC Class Codes: B01J 20/06 comprising oxides or hydroxides of metals not provided for in group B01J20/04
• CPC Class Codes: B01J 20/08 comprising aluminium oxide or hydroxide; comprising bauxite
• CPC Class Codes: B01J 20/103 comprising silica
• CPC Class Codes: B01J 20/28061 being in the range 100-500 m2/g
• CPC Class Codes: B01J 20/3078 Thermal treatment, e.g. calcining or pyrolizing
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/3293 Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
• CPC Class Codes: B01J 20/3297 Coatings in the shape of a sheet
• CPC Class Codes: B01J 2220/42 Materials comprising a mixture of inorganic materials materials coated or impregnated on a carrier B01J20/32
• CPC Class Codes: B01J 2220/58 Use in a single column
• CPC Class Codes: C02F 1/286 using natural organic sorbents or derivatives thereof
• CPC Class Codes: C02F 1/288 using composite sorbents, e.g. coated, impregnated, multi-layered
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

A DNA hybrid which comprises a porous oxide matrix and DNA immobilized thereon, and is useful for environmental clean-up, where the hybrid is prepared by removing a dispersion medium from a dispersion of colloidal oxide and DNA.

Claims

1. A method for producing a DNA hybrid comprising the steps of:

preparing a dispersion comprising colloidal silica, at least one colloidal metal oxide which comprises an oxide of a metal with a valence of 3 or 4, and a DNA, wherein the colloidal silica and the at least one colloidal metal oxide are separate components of the dispersion; and

gelling the dispersion by heating to immobilize the DNA in a porous matrix made by gellation of the colloidal silica and the at least one colloidal metal oxide,

wherein the content of the 3- or 4-valent metal oxide is 0.1 to 50% by mass relative to the total mass of the colloidal silica and the at least one colloidal metal oxide.

2. The method according to claim 1, wherein the gellation of the colloidal silica and the at least one colloidal metal oxide is effected by removing a dispersion medium by heating the dispersion at 25°

C. to 200°

C.

3. The method according to claim 1, wherein the content of the DNA is 0.01 to 15% by mass relative to the total mass of the DNA hybrid.

4. The method according to claim 1, wherein the at least one colloidal metal oxide comprises at least one oxide selected from the group consisting of aluminum oxide, iron oxide, titanium oxide and zirconium oxide.